Li Pengfei, He Qiyu, Jin Jianfeng, Liu Yu, Wen Yuxin, Zhao Kai, Mao Guangqun, Fan Wei, Yang Jianli
State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, China.
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.
Front Plant Sci. 2022 Jul 7;13:951386. doi: 10.3389/fpls.2022.951386. eCollection 2022.
Acyl activating enzyme 3 (AAE3) encodes oxalyl-CoA synthetase involved in oxalate degradation. In this study, we investigated the role of AAE3 (SlAAE3) in the fruit quality of tomato (). The purified recombinant SlAAE3 protein from exhibited a high activity toward oxalate, with a K of 223.8 ± 20.03 μm and V of 7.908 ± 0.606 μmol mg protein min. Transient expression of SlAAE3-green fluorescent protein (GFP) fusion proteins suggests that SlAAE3 is a soluble protein without specific subcellular localization. The expression of SlAAE3 is both tissue- and development-dependent, and increased during fruit ripping. The knockout mutants had improved fruit quality as evidenced by the increased sugar-acid ratio and mineral nutrient content. To find the mechanism by which SlAAE3 affects fruit quality, transcriptome, and metabolome were employed on SlAAE3 over-expressed line and wide type fruits. The transcriptomic and metabolic profiles indicated that SlAAE3 in fruits mainly functions at 20 days post-anthesis (20 DPA) and mature green (MG) stages, resulting in up-regulation of amino acid derivatives, nucleotides, and derivatives, but down-regulation of lipid compounds. However, differentially expressed genes (DEGs) were mainly enriched at redox pathways. Taken together, both and results suggest that SlAAE3-encoded protein acts as an oxalyl-CoA synthetase, which also participates in redox metabolism. These data provide a further understanding of the mechanism by which SlAAE3 participates in tomato fruit quality.
酰基激活酶3（AAE3）编码参与草酸盐降解的草酰辅酶A合成酶。在本研究中，我们调查了AAE3（SlAAE3）在番茄果实品质中的作用。从[具体来源未明确]纯化得到的重组SlAAE3蛋白对草酸盐表现出高活性，其米氏常数（K）为223.8±20.03μM，最大反应速度（V）为7.908±0.606μmol·mg蛋白⁻¹·min⁻¹。SlAAE3-绿色荧光蛋白（GFP）融合蛋白的瞬时表达表明SlAAE3是一种可溶性蛋白，无特定亚细胞定位。SlAAE3的表达既依赖于组织也依赖于发育阶段，并且在果实成熟过程中增加。[具体基因名称未明确]敲除突变体的果实品质得到改善，糖酸比和矿质营养含量增加证明了这一点。为了找到SlAAE3影响果实品质的机制，对SlAAE3过表达株系和野生型果实进行了转录组和代谢组分析。转录组和代谢谱表明，果实中的SlAAE3主要在花后20天（20 DPA）和绿熟期（MG）发挥作用，导致氨基酸衍生物、核苷酸及其衍生物上调，但脂质化合物下调。然而，差异表达基因（DEGs）主要富集在氧化还原途径。综上所述，[具体实验名称未明确]和[具体实验名称未明确]的结果表明，SlAAE3编码的蛋白作为草酰辅酶A合成酶，也参与氧化还原代谢。这些数据进一步阐明了SlAAE3参与番茄果实品质形成的机制。
