Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, Zhejiang 310003, China.
Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, Zhejiang 310003, China.
STAR Protoc. 2022 Jul 18;3(3):101559. doi: 10.1016/j.xpro.2022.101559. eCollection 2022 Sep 16.
Acute myeloid leukemia (AML) is one of the most common and fatal forms of hematopoietic malignancies. Here, we describe a mouse _AML model to investigate AML. We have optimized the protocols for retrovirus infection, bone marrow transplantation (BMT), and leukemia monitoring to create a stable mouse model. In particular, we have used two rounds of BMT to enhance stability and efficiency. This model can be used to conduct drug administration and/or other interventions easily. For complete details on the use and execution of this protocol, please refer to Zhao et al. (2022).
急性髓系白血病（AML）是最常见和致命的血液系统恶性肿瘤之一。在这里，我们描述了一种用于研究 AML 的小鼠 AML 模型。我们已经优化了逆转录病毒感染、骨髓移植（BMT）和白血病监测的方案，以创建一个稳定的小鼠模型。特别是，我们使用了两轮 BMT 来提高稳定性和效率。该模型可用于方便地进行药物给药和/或其他干预。有关此方案的使用和执行的完整详细信息，请参阅 Zhao 等人（2022 年）。
