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荧光标记的组成型表达eGFP、eCFP或dsRED的DC3000和1449b野生型菌株。

Fluorescently labeled DC3000 and 1449b wild-type strains constitutively expressing either eGFP, eCFP, or dsRED.

作者信息

Rufián Jose S, Ruiz-Albert Javier, Beuzón Carmen R

机构信息

Departamento Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC).

出版信息

MicroPubl Biol. 2022 Jul 6;2022. doi: 10.17912/micropub.biology.000595. eCollection 2022.

DOI:10.17912/micropub.biology.000595
PMID:35874602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9297101/
Abstract

Here we describe the generation of fluorescently labeled derivatives of the plant pathogen DC3000 and 1449b strains, with each derivative constitutively expressing either the enhanced green (eGFP), enhanced cyan (eCFP), or sp. red (dsRED) fluorescent proteins. The fluorophore-expressing cassetes are stably located in a neutral locus in the chromosome, and its expression does not affect bacterial fitness, while allowing efficient detection by microscopy or flow cytometry. We have generated these strains as a complementary set of labeled strains to those previously generated in our laboratory, thus extending the range of applications.

摘要

在此，我们描述了植物病原体DC3000和1449b菌株的荧光标记衍生物的产生，每个衍生物组成型表达增强型绿色（eGFP）、增强型青色（eCFP）或sp.红色（dsRED）荧光蛋白。表达荧光团的盒式结构稳定地位于染色体的一个中性位点，其表达不影响细菌适应性，同时允许通过显微镜或流式细胞术进行有效检测。我们已生成这些菌株，作为我们实验室之前生成的标记菌株的补充菌株集，从而扩展了应用范围。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2530/9297101/3fbe7e0fb582/25789430-2022-micropub.biology.000595.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2530/9297101/3fbe7e0fb582/25789430-2022-micropub.biology.000595.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2530/9297101/3fbe7e0fb582/25789430-2022-micropub.biology.000595.jpg

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Confocal microscopy reveals in planta dynamic interactions between pathogenic, avirulent and non-pathogenic Pseudomonas syringae strains.共聚焦显微镜揭示了植物体内致病性、无毒和非致病性丁香假单胞菌菌株之间的动态相互作用。
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