Utami Dian, Chalid Maisuri T, Masadah Rina, Sjahril Rizalinda, Bahagia Febriani Andi Dwi
Department of Obstetrics and Gynecology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Interdiscip Perspect Infect Dis. 2022 Jul 13;2022:4838376. doi: 10.1155/2022/4838376. eCollection 2022.
The placenta is a specialized organ that only performs during pregnancy and serves as an immunological barrier in preventing pathogens to reach the fetus. It has been known that toll-like receptors (TLRs) on the placenta respond to antigens, such as zymosan, lipopolysaccharide, and other viral infections. This study analyzes the distribution of TLR2 protein and hepatitis B DNA virus (HBV DNA) virus identification to gain an understanding of hepatitis B viral transmission from the mother to child.
We performed enzyme-linked immunoadsorbent assay of HBeAg, HBsAg titer, Anti-HBs, and Anti-HBc to 59 HBsAg-positive pregnant women and identified HBV DNA using nested PCR in their cord blood during delivery for evidence of exposure to HBV DNA. The expression of TLR2 protein in the placenta was performed using immunohistochemistry analysis.
Intrauterine exposure to the hepatitis B virus occurred in 69.5% of all pregnant women who were HbsAg positive. TLR2 expression was predominantly identified in syncytiotrophoblast and cytotrophoblast cells with the highest score in mothers aged 20-35 years (75%), multigravida (58.3%), and mothers with term pregnancies (70.8%). Statistical analysis results showed that placental TLR2 expression did not indicate any association with hepatitis B virus DNA identified in cord blood with a value of 0.730 and an OR of 0.650 (95% CI 0.173-2.440).
TLR2 expression is not associated with intrauterine exposure of hepatitis B virus.
胎盘是一个仅在孕期发挥作用的特殊器官，它作为一道免疫屏障，可防止病原体感染胎儿。已知胎盘上的Toll样受体（TLRs）能对抗原产生反应，如酵母聚糖、脂多糖及其他病毒感染。本研究分析了TLR2蛋白的分布及乙肝DNA病毒鉴定情况，以了解乙肝病毒的母婴传播。
我们对59例HBsAg阳性孕妇进行了HBeAg、HBsAg滴度、抗-HBs及抗-HBc的酶联免疫吸附测定，并在分娩时采集其脐血，采用巢式PCR鉴定HBV DNA，以证明是否存在HBV DNA暴露。采用免疫组织化学分析法检测胎盘组织中TLR2蛋白的表达。
所有HBsAg阳性孕妇中，69.5%发生了宫内乙肝病毒暴露。TLR2表达主要见于合体滋养层细胞和细胞滋养层细胞，年龄在20 - 35岁的母亲（75%）、经产妇（58.3%）及足月妊娠母亲（70.8%）的TLR2表达得分最高。统计分析结果显示，胎盘TLR2表达与脐血中鉴定出的乙肝病毒DNA无相关性，P值为0.730，OR为0.650（95%CI 0.173 - 2.440）。
TLR2表达与乙肝病毒宫内暴露无关。
