Key Laboratory of Urban Environment and Health, Ningbo Urban Environment Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China.
Environ Sci Technol. 2022 Nov 1;56(21):15034-15043. doi: 10.1021/acs.est.2c02471. Epub 2022 Jul 23.
Multitrophic communities inhabit in soil faunal gut, including bacteria, fungi, and protists, which have been considered a hidden reservoir for antibiotic resistance genes (ARGs). However, there is a dearth of research focusing on the relationships between ARGs and multitrophic communities in the gut of soil faunas. Here, we studied the contribution of multitrophic communities to variations of ARGs in the soil woodlouse gut. The results revealed diverse and abundant ARGs in the woodlouse gut. Network analysis further exhibited strong connections between key ecological module members and ARGs, suggesting that multitrophic communities in the keystone ecological cluster may play a pivotal role in the variation of ARGs in the woodlouse gut. Moreover, long-term application of sewage sludge significantly altered the woodlice gut resistome and interkingdom communities. The variation portioning analysis indicated that the fungal community has a greater contribution to variations of ARGs than bacterial and protistan communities in the woodlice gut after long-term application of sewage sludge. Together, our results showed that changes in gut microbiota associated with agricultural practices (e.g., sewage sludge application) can largely alter the gut interkingdom network in ecologically relevant soil animals, with implications for antibiotic resistance, which advances our understanding of the microecological drivers of ARGs in terrestrial ecosystem.
土壤动物肠道中的后生动物肠道中栖息着多种营养级的群落，包括细菌、真菌和原生生物，它们被认为是抗生素耐药基因（ARGs）的隐藏库。然而，目前针对土壤动物肠道中 ARGs 与多营养级群落之间关系的研究还很少。在这里，我们研究了多营养级群落对土壤潮虫肠道中 ARGs 变化的贡献。结果表明，潮虫肠道中存在多样且丰富的 ARGs。网络分析进一步显示了关键生态模块成员与 ARGs 之间的紧密联系，表明在关键生态聚类中的多营养级群落可能在潮虫肠道中 ARGs 的变化中发挥关键作用。此外，长期施用污水污泥显著改变了潮虫肠道的抗药性和种间群落。变分分析表明，与长期施用污水污泥后细菌和原生生物群落相比，真菌群落对潮虫肠道中 ARGs 的变化有更大的贡献。总之，我们的研究结果表明，与农业实践（例如，污水污泥的应用）相关的肠道微生物群的变化可以极大地改变在生态上相关的土壤动物的肠道种间网络，这对了解陆地生态系统中抗生素耐药性的微观生态驱动因素具有重要意义。
