Departments of Microbiology and Immunology, Indiana University, Indianapolis, Indiana, USA.
Department of Medicine, Indiana University, Indianapolis, Indiana, USA.
J Infect Dis. 2023 Feb 1;227(3):317-321. doi: 10.1093/infdis/jiac314.
Compared with wounded skin, ascorbic acid is enriched in pustules of humans experimentally infected with Haemophilus ducreyi. Compared with the broth-grown inocula, transcription of the H. ducreyi ulaABCD operon, which encodes genes for ascorbic acid uptake, is increased in pustules. We hypothesized that ascorbic acid uptake plays a role in H. ducreyi virulence. Five volunteers were infected with both H. ducreyi strain 35000HP and its isogenic ulaABCD deletion mutant at multiple sites; the papule and pustule formation rates of the mutant and parent strains were similar. Thus, ascorbic acid uptake is not essential for H. ducreyi virulence in humans.
与创伤皮肤相比,在人类感染杜克嗜血杆菌的脓疱中富含抗坏血酸。与在肉汤中生长的接种物相比,编码抗坏血酸摄取的基因的 H. ducreyi ulaABCD 操纵子的转录在脓疱中增加。我们假设抗坏血酸摄取在 H. ducreyi 毒力中起作用。五名志愿者在多个部位同时感染了 H. ducreyi 菌株 35000HP 及其同源的 ulaABCD 缺失突变体;突变体和亲本菌株的丘疹和脓疱形成率相似。因此,在人类中,抗坏血酸摄取对 H. ducreyi 毒力不是必需的。