维生素 C 摄取和利用所需 ulaABCD 编码对杜克雷嗜血菌人类毒力的必要性。
Dispensability of Ascorbic Acid Uptake and Utilization Encoded by ulaABCD for the Virulence of Haemophilus ducreyi in Humans.
机构信息
Departments of Microbiology and Immunology, Indiana University, Indianapolis, Indiana, USA.
Department of Medicine, Indiana University, Indianapolis, Indiana, USA.
出版信息
J Infect Dis. 2023 Feb 1;227(3):317-321. doi: 10.1093/infdis/jiac314.
Abstract
Compared with wounded skin, ascorbic acid is enriched in pustules of humans experimentally infected with Haemophilus ducreyi. Compared with the broth-grown inocula, transcription of the H. ducreyi ulaABCD operon, which encodes genes for ascorbic acid uptake, is increased in pustules. We hypothesized that ascorbic acid uptake plays a role in H. ducreyi virulence. Five volunteers were infected with both H. ducreyi strain 35000HP and its isogenic ulaABCD deletion mutant at multiple sites; the papule and pustule formation rates of the mutant and parent strains were similar. Thus, ascorbic acid uptake is not essential for H. ducreyi virulence in humans.
摘要
与创伤皮肤相比，在人类感染杜克嗜血杆菌的脓疱中富含抗坏血酸。与在肉汤中生长的接种物相比，编码抗坏血酸摄取的基因的 H. ducreyi ulaABCD 操纵子的转录在脓疱中增加。我们假设抗坏血酸摄取在 H. ducreyi 毒力中起作用。五名志愿者在多个部位同时感染了 H. ducreyi 菌株 35000HP 及其同源的 ulaABCD 缺失突变体；突变体和亲本菌株的丘疹和脓疱形成率相似。因此，在人类中，抗坏血酸摄取对 H. ducreyi 毒力不是必需的。
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