建立并鉴定 IPS-OGC-C1：一种来自健康人卵巢颗粒细胞的新型诱导多能干细胞系。
Establishment and characterization of IPS-OGC-C1: a novel induced pluripotent stem cell line from healthy human ovarian granulosa cells.
机构信息
Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China.
Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
出版信息
Hum Cell. 2022 Sep;35(5):1612-1620. doi: 10.1007/s13577-022-00757-7. Epub 2022 Jul 25.
Ovarian granulosa cell (OGC) is a critical somatic component of the ovary, which provides physical support and the microenvironment required for the developing oocyte. Human OGCs are easy to obtain and culture as a by-product of follicular aspiration performed during in vitro fertilization (IVF) procedures. Therefore, OGCs offer a potent cell source to generate induced pluripotent stem cells (iPSCs). This study established a novel OGCs-derived iPSC cell line from the follicular fluid of a healthy female donor with a Chinese Han genetic background and named it IPS-OGC-C1. IPS-OGC-C1 was verified for embryonic stem cell morphology, cell marker expression, alkaline phosphatase (AP) activity, transcriptomic profile, and pluripotency capability in developing all three embryonic germ layers in vivo and in vitro.
卵巢颗粒细胞（OGC）是卵巢的重要体细胞成分，为发育中的卵母细胞提供物理支持和微环境。人卵巢颗粒细胞易于获得和培养，可作为体外受精（IVF）过程中卵泡抽吸的副产品。因此，OGC 是生成诱导多能干细胞（iPSC）的有效细胞来源。本研究从一名中国汉族健康女性供体的卵泡液中建立了一种新型的 OGC 来源的 iPSC 细胞系，并将其命名为 IPS-OGC-C1。通过胚胎干细胞形态、细胞标志物表达、碱性磷酸酶（AP）活性、转录组谱以及体内和体外发育成三个胚胎胚层的多能性能力对 IPS-OGC-C1 进行了验证。
Zotero 插件