Sievers J, Hausmann B, Unsicker K, Berry M
Neurosci Lett. 1987 May 6;76(2):157-62. doi: 10.1016/0304-3940(87)90708-7.
Basic and acidic fibroblast growth factors (FGF) were implanted next to the proximal stump of the transected optic nerve of adult rats, in order to assess whether these molecules have neurotrophic activity in vivo. Of the 119,973 +/- 2484 (S.E.M.) retinal ganglion cells present in retinae of unoperated control rats, 11,375 +/- 2413 (S.E.M.) remained at 30 days after transection of the optic nerve in control operated rats. After implantation of gel foam soaked in basic FGF, the number of retinal ganglion cells surviving at 30 days after axotomy tripled (36,387 +/- 3270 (S.E.M.], after acidic FGF, it increased almost 4-fold (40,916 +/- 5405 (S.E.M.]. These results indicate that FGF has neurotrophic activity in the adult central nervous system, and that this molecule is able to rescue adult retinal ganglion cells from axotomy induced cell death. It remains to be shown whether FGF acts directly on retinal ganglion cells or indirectly via glial cells or other cells.
将碱性和酸性成纤维细胞生长因子（FGF）植入成年大鼠横断视神经近端残端旁，以评估这些分子在体内是否具有神经营养活性。在未手术对照大鼠视网膜中存在的119,973±2484（标准误）个视网膜神经节细胞中，对照手术大鼠视神经横断后30天，有11,375±2413（标准误）个细胞存活。植入浸泡有碱性FGF的明胶海绵后，轴突切断后30天存活的视网膜神经节细胞数量增加了两倍（36,387±3270（标准误）），植入酸性FGF后，数量增加了近四倍（40,916±5405（标准误））。这些结果表明，FGF在成年中枢神经系统中具有神经营养活性，并且该分子能够挽救成年视网膜神经节细胞免于轴突切断诱导的细胞死亡。FGF是直接作用于视网膜神经节细胞还是通过神经胶质细胞或其他细胞间接作用，仍有待证明。
