Department of Clinical Experimental Science and Odontostomatology-Biochemistry, Università Politecnica delle Marche, 60100 Ancona, Italy.
Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy.
Mar Drugs. 2022 Jul 14;20(7):457. doi: 10.3390/md20070457.
(L.) Delile is an endemic Mediterranean marine plant of extreme ecological importance. Previous in vitro and in vivo studies have demonstrated the potential antidiabetic properties of leaf extract. Intestinal glucose transporters play a key role in glucose homeostasis and represent novel targets for the management of diabetes. In this study, the ability of a hydroalcoholic leaf extract (POE) to modulate intestinal glucose transporters was investigated using Caco-2 cells as a model of an intestinal barrier. The incubation of cells with POE significantly decreased glucose uptake by decreasing the GLUT2 glucose transporter levels. Moreover, POE had a positive effect on the barrier integrity by increasing the Zonulin-1 levels. A protective effect exerted by POE against oxidative stress induced by chronic exposure to high glucose concentrations or tert-butyl hydroperoxide was also demonstrated. This study highlights for the first time the effect of POE on glucose transport, intestinal barrier integrity, and its protective antioxidant effect in Caco-2 cells. These findings suggest that the phytocomplex may have a positive impact by preventing the intestinal cell dysfunction involved in the development of inflammation-related disease associated with oxidative stress.
（L.） Delile 是一种地中海特有的海洋植物，具有极端重要的生态意义。先前的体外和体内研究表明，该植物的叶子提取物具有潜在的抗糖尿病特性。肠道葡萄糖转运蛋白在葡萄糖稳态中起着关键作用，是糖尿病治疗的新靶点。在这项研究中，使用 Caco-2 细胞作为肠道屏障模型，研究了一种水醇叶提取物（POE）对肠道葡萄糖转运蛋白的调节能力。细胞与 POE 孵育后，通过降低 GLUT2 葡萄糖转运蛋白水平，显著降低了葡萄糖摄取。此外，POE 通过增加 Zonulin-1 水平对屏障完整性产生了积极影响。还证明了 POE 对慢性暴露于高葡萄糖浓度或叔丁基过氧化物引起的氧化应激具有保护作用。这项研究首次强调了 POE 对葡萄糖转运、肠道屏障完整性以及其在 Caco-2 细胞中的抗氧化保护作用的影响。这些发现表明，这种植物复合物可能通过预防与氧化应激相关的炎症性疾病发展过程中涉及的肠道细胞功能障碍，产生积极影响。
