为什么在培养皿中研究人猴嵌合体胚胎很重要。
Why it is important to study human-monkey embryonic chimeras in a dish.
机构信息
Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA.
Johns Hopkins Berman Institute of Bioethics, Johns Hopkins University, Baltimore, MD, USA.
出版信息
Nat Methods. 2022 Aug;19(8):914-919. doi: 10.1038/s41592-022-01571-7.
Abstract
The study of human–animal chimeras is fraught with technical and ethical challenges. In this Comment, we discuss the importance and future of human–monkey chimera research within the context of current scientific and regulatory obstacles.
摘要
人类-动物嵌合体的研究充满了技术和伦理挑战。在这篇评论中，我们讨论了在当前科学和监管障碍的背景下，人类-猴嵌合体研究的重要性和未来。
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2
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3
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4
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