Institute for Molecular Bioscience, The University of Queensland, Brisbane, 4072, Australia.
Institute of Biological Chemistry, University of Vienna, Währingerstraße 38, Vienna, 1090, Austria.
Chem Commun (Camb). 2022 Aug 11;58(65):9100-9103. doi: 10.1039/d2cc03752a.
The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min.
肟连接是一种有价值的生物正交共轭反应，但与富含二硫键的肽/蛋白质的兼容性有限，并且不适合时间敏感的应用。在这里，我们通过引入一种支持区域特异性控制、氧化折叠、生成稳定的氨氧基前体以实现按需修饰以及在 5 分钟内完成完全连接的策略克服了这些限制。
