Hu Chunlin, Xie Jing
College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
National Experimental Teaching Demonstration Center for Food Science and Engineering, Shanghai Ocean University, Shanghai, China.
J Food Sci. 2022 Sep;87(9):3938-3952. doi: 10.1111/1750-3841.16209. Epub 2022 Jul 26.
We investigated the proteome variations in Trachurus murphyi with different cycles of freezing and thawing (FT) under frozen storage. A total of 2,482 proteins were assessed quantitatively, of which 269 proteins were recognized as differential abundance proteins during the second FT cycle until the eighth FT cycle. Bioinformatics analysis on gene ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway analyses of Differential Analysis of Proteins (DAPs) indicated multiple DAPs engaged with the protein structure, metabolic enzymes, and protein turnover. In addition, some of the observed proteins were probably the underlying markers of protein oxidation (PO). The analysis of PO sites revealed the sites of PO, such as amino adipic semialdehydes, γ-glutamic semialdehydes, and Schiff bases. Bioinformatics analyses demonstrated the involvement of differentially expressed proteins in the Hippo signaling pathway (Ko04390), indicating strong protein degradation with greater numbers of FT cycles under frozen storage. It provides an insight into quality stability from a proteomics quality perspective at the molecular level. The results obtained have deepened our current understandings of the mechanisms that reveal variations in proteomes and quality, as well as help promote quality control of T. murphyi across the cold transportation chain. PRACTICAL APPLICATION: Temperature fluctuation is one of the core issues during frozen food storage and distribution faced by the frozen food industry. Fluctuation may result in microstructural changes, ice recrystallization, and protein change in frozen food products. Tandem mass tag-based methods were adopted to study proteome variations in Trachurus murphyi muscles under different cycles of freezing and thawing under frozen storage conditions in this paper. The results obtained have deepened our current understandings of the mechanisms that reveal variations in proteomes and quality, as well as help promote quality control of T. murphyi across the cold transportation chain.
我们研究了在冷冻储存条件下，不同冻融（FT）循环次数的智利竹筴鱼的蛋白质组变化。共对2482种蛋白质进行了定量评估，其中269种蛋白质在第二次FT循环至第八次FT循环期间被识别为差异丰度蛋白质。对差异分析蛋白质（DAPs）进行基因本体（GO）功能注释和京都基因与基因组百科全书通路分析的生物信息学分析表明，多个DAPs与蛋白质结构、代谢酶和蛋白质周转有关。此外，一些观察到的蛋白质可能是蛋白质氧化（PO）的潜在标志物。PO位点分析揭示了PO的位点，如氨基己二酸半醛、γ-谷氨酸半醛和席夫碱。生物信息学分析表明，差异表达的蛋白质参与了Hippo信号通路（Ko04390），表明在冷冻储存条件下，随着FT循环次数的增加，蛋白质降解加剧。这从蛋白质组学质量的分子水平为质量稳定性提供了见解。所获得的结果加深了我们目前对揭示蛋白质组和质量变化机制的理解，并有助于促进智利竹筴鱼在冷链运输过程中的质量控制。实际应用：温度波动是冷冻食品行业在冷冻食品储存和配送过程中面临的核心问题之一。波动可能导致冷冻食品产品的微观结构变化、冰重结晶和蛋白质变化。本文采用基于串联质量标签的方法研究了在冷冻储存条件下，不同冻融循环次数的智利竹筴鱼肌肉中的蛋白质组变化。所获得的结果加深了我们目前对揭示蛋白质组和质量变化机制的理解，并有助于促进智利竹筴鱼在冷链运输过程中的质量控制。
