Zhou Yingqun, Chen Jiaojiao, Yao Zhilu, Gu Xuezhen
Department of Gastroenterology, Shanghai Tenth People's Hospital, Nanjing Medical, University, Shanghai, China.
Department of Gastroenterology, Shanghai Tenth People's Hospital, Chongming Branch, Shanghai, China.
Immunopharmacol Immunotoxicol. 2022 Dec;44(6):925-934. doi: 10.1080/08923973.2022.2093741. Epub 2022 Jul 26.
Gastrodin, the main active ingredient of Gastrodia elata Blume, has been shown to protect against many inflammatory diseases. Our study aimed to investigate the anti-inflammatory role of gastrodin in concanavalin A (ConA)-induced acute hepatitis in mice and to explore its precise mechanism.
C57BL/6 mice were administered with gastrodin (50 or 100mg/kg) for 3 days prior to intravenous injection of ConA to induce acute autoimmune hepatitis (AIH). Serum aminotransferases levels and cytokine levels were measured. Liver tissue histology was conducted to assess the degree of liver injury. Splenocytes pretreated with gastrodin were stimulated with ConA to observe splenocyte proliferation.
Gastrodin greatly reduced the level of serum aminotransferases, inflammatory cytokine such as IL-6 and TNF-α and histopathological damage in ConA-induced hepatitis. Besides, gastrodin had an inhibitory effect on liver apoptosis, and autophagy. Furthermore, gastrodin inhibited the proliferation of splenocytes in vitro. The protein expression of p-JAK2 and p-STAT3 was markedly affected by gastrodin pretreatment.
The present study indicated that gastrodin pretreatment exerted protective effects against ConA-induced acute hepatitis, partly through the inhibition of the IL6/JAK2/STAT3 pathway. Further studies are recommended to determine the potential therapeutic role of gastrodin in acute AIH.
天麻素是天麻的主要活性成分，已被证明可预防多种炎症性疾病。我们的研究旨在探讨天麻素在刀豆蛋白A（ConA）诱导的小鼠急性肝炎中的抗炎作用，并探索其确切机制。
在静脉注射ConA诱导急性自身免疫性肝炎（AIH）之前，给C57BL/6小鼠连续3天给予天麻素（50或100mg/kg）。检测血清转氨酶水平和细胞因子水平。进行肝组织组织学检查以评估肝损伤程度。用天麻素预处理脾细胞，然后用ConA刺激以观察脾细胞增殖。
天麻素可显著降低ConA诱导的肝炎中血清转氨酶水平、炎性细胞因子如IL-6和TNF-α水平以及组织病理学损伤。此外，天麻素对肝脏细胞凋亡和自噬有抑制作用。此外，天麻素在体外抑制脾细胞增殖。天麻素预处理显著影响p-JAK2和p-STAT3的蛋白表达。
本研究表明，天麻素预处理对ConA诱导的急性肝炎具有保护作用，部分是通过抑制IL6/JAK2/STAT3途径实现的。建议进一步研究以确定天麻素在急性AIH中的潜在治疗作用。
