竞争 ELISA 分析抗体对 PfEMP1 结合的抑制作用。
Analysis of Antibody Inhibition of PfEMP1 Binding by Competition ELISA.
机构信息
Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark.
Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
出版信息
Methods Mol Biol. 2022;2470:485-491. doi: 10.1007/978-1-0716-2189-9_36.
Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.
恶性疟原虫在感染的红细胞（IEs）表面表达变异抗原。这些抗原包括恶性疟原虫红细胞膜蛋白 1（PfEMP1）家族的蛋白质。PfEMP1 蛋白介导 IEs 与各种人类内皮受体的结合，并在自然感染过程中诱导抗体反应。在本章中，我们描述了一种竞争 ELISA，这是一种用于检测中和 PfEMP1 蛋白与其同源受体结合的抗体的检测方法。
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