Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Biosensors (Basel). 2022 Jun 26;12(7):458. doi: 10.3390/bios12070458.
Food poisoning and infectious diseases caused by () are serious public health concerns for human health and food safety. The diversity and complexity of food matrices pose great challenges for rapid and ultra-sensitive detection of in food samples. A method capable of identification, detection, and quantification of is essential for addressing these issues. In this study, aptamer-coated magnetic beads (Apt-MBs) are employed as capture bio-probes to specifically and selectively concentrate in food samples. A self-priming chip-based digital PCR was then presented as another biosensor for on-site detection and quantification of cells. The chip we developed was robust and did not require any external power for sample loading. The combination of Apt-MBs with an on-chip digital detection realized the integration into lab-on-a-chip-based biosensors for on-site monitoring of foodborne pathogens. It was possible to capture and detect cells as low as 90 CFU/reaction with a capture efficiency of 94.5%. Additionally, the whole process only took about 2 h. This unique platform could also be used to monitor other target bacteria with high specificity and sensitivity by utilizing different aptamers. Furthermore, the platform has potential applications in point-of-care testing in the future.
（）引起的食物中毒和传染病对人类健康和食品安全构成严重的公共卫生关注。食物基质的多样性和复杂性对食物样本中进行快速和超敏检测（）带来了巨大挑战。能够识别、检测和定量（）的方法对于解决这些问题至关重要。在本研究中，适配体包被的磁性珠（Apt-MBs）被用作捕获生物探针，以特异性和选择性地浓缩食物样本中的（）。然后，提出了一种自启动芯片基数字 PCR 作为另一种生物传感器，用于现场检测和定量（）细胞。我们开发的芯片坚固耐用，无需外部电源即可进行样品加载。Apt-MBs 与芯片上的数字检测相结合，实现了基于微流控芯片的生物传感器的集成，用于现场监测食源性病原体。可以捕获和检测低至 90 CFU/反应的（）细胞，捕获效率为 94.5%。此外，整个过程仅需约 2 小时。通过利用不同的适配体，该平台还可以用于监测其他具有高特异性和灵敏度的目标细菌。此外，该平台将来有可能在即时检测中得到应用。
