发育阶段对玫瑰花瓣中挥发性萜类生物合成中下游途径相关基因的影响。

Effect of Developmental Stages on Genes Involved in Middle and Downstream Pathway of Volatile Terpene Biosynthesis in Rose Petals.

机构信息

Institute of Radiation Technology, Beijing Academy of Science and Technology, Beijing 100875, China.

Key Lab of Beam Technology and Material Modification of Ministry of Education, College of Nuclear Science and Technology, Beijing Normal University, Beijing 100875, China.

出版信息

Genes (Basel). 2022 Jun 30;13(7):1177. doi: 10.3390/genes13071177.

DOI:10.3390/genes13071177

PMID:35885960

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9320630/

Abstract

Terpenoids are economically and ecologically important compounds, and they are vital constituents in rose flower fragrance and rose essential oil. The terpene synthase genes (TPSs), -prenyltransferases genes (TPTs), are involved in middle and downstream pathway of volatile terpene biosynthesis in rose flowers. We identified 7 complete s, 49 complete s, and 9 genes in the genome of . During the flower opening process of butterfly rose ( 'Mutabilis', MU), nine expressed in the petals of opening MU flowers exhibited two main expression trends, namely high and low, in old and fresh petals. Five short-chain petal-expressed showed expression patterns corresponding to s. Analysis of differential volatile terpenes and differential expressed genes indicated that higher emission of geraniol from old MU petals might be related to the expression. Comprehensive analysis of volatile emission, sequence structure, micro-synteny and gene expression suggested that may encode (,)-α-farnesene synthase. These findings may be useful for elucidating the molecular mechanism of terpenoid metabolism in rose and are vital for future studies on terpene regulation.

摘要

萜类化合物具有重要的经济和生态意义，是玫瑰花香味和玫瑰精油的重要组成部分。萜烯合酶基因（TPS）和 - 异戊烯基转移酶基因（TPT）参与了玫瑰花挥发性萜烯生物合成的中下游途径。我们在月季（‘Mutabilis’，MU）基因组中鉴定出 7 个完整的 TPS 基因、49 个完整的 TPT 基因和 9 个基因。在蝴蝶月季（'Mutabilis'，MU）的开花过程中，在开放 MU 花瓣中表达的 9 个基因表现出两种主要的表达趋势，即老花瓣和新花瓣中的高和低。5 个短链花瓣表达的基因表现出与 s 基因表达模式相对应的表达模式。对差异挥发性萜类化合物和差异表达基因的分析表明，老 MU 花瓣中香叶醇的高释放可能与基因的表达有关。挥发性物质排放、序列结构、微共线性和基因表达的综合分析表明，可能编码（，）-α-法呢烯合酶。这些发现可能有助于阐明玫瑰萜类代谢的分子机制，对未来的萜类调节研究至关重要。