Department of Plants Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Department of Gynecology and Obstetrics, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-561 Olsztyn, Poland.
Int J Mol Sci. 2022 Jul 11;23(14):7642. doi: 10.3390/ijms23147642.
Long non-coding RNAs (lncRNAs) are transcripts not translated into proteins with a length of more than 200 bp. LncRNAs are considered an important factor in the regulation of countless biological processes, mainly through the regulation of gene expression and interactions with proteins. However, the detailed mechanism of interaction as well as functions of lncRNAs are still unclear and therefore constitute a serious research challenge. In this study, for the first time, potential mechanisms of lncRNA regulation of processes related to sperm motility in turkey were investigated and described. Customized bioinformatics analysis was used to detect and identify lncRNAs, and their correlations with differentially expressed genes and proteins were also investigated. Results revealed the expression of 863 new/unknown lncRNAs in ductus deferens, testes and epididymis of turkeys. Moreover, potential relationships of the lncRNAs with the coding mRNAs and their products were identified in turkey reproductive tissues. The results obtained from the OMICS study may be useful in describing and characterizing the way that lncRNAs regulate genes and proteins as well as signaling pathways related to sperm motility.
长链非编码 RNA（lncRNA）是一种不翻译成蛋白质的转录物，长度超过 200bp。lncRNA 被认为是调节无数生物过程的重要因素，主要通过调节基因表达和与蛋白质的相互作用。然而，lncRNA 相互作用的详细机制及其功能仍不清楚，因此构成了一个严重的研究挑战。在这项研究中，首次研究并描述了 lncRNA 对火鸡精子运动相关过程的调节潜在机制。使用定制的生物信息学分析来检测和识别 lncRNA，还研究了它们与差异表达基因和蛋白质的相关性。结果显示，在火鸡的输精管、睾丸和附睾中表达了 863 种新的/未知的 lncRNA。此外，还在火鸡生殖组织中鉴定了 lncRNA 与编码 mRNA 及其产物的潜在关系。从 OMICS 研究中获得的结果可能有助于描述和表征 lncRNA 调节基因和蛋白质以及与精子运动相关的信号通路的方式。
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