Fraser Leyland, Paukszto Łukasz, Mańkowska Anna, Brym Paweł, Gilun Przemysław, Jastrzębski Jan P, Pareek Chandra S, Kumar Dibyendu, Pierzchała Mariusz
Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Life (Basel). 2020 Nov 21;10(11):300. doi: 10.3390/life10110300.
Long non-coding RNAs (lncRNAs) are suggested to play an important role in the sperm biological processes. We performed transcriptome assembly to characterize lncRNAs in spermatozoa, and to investigate the role of the potential target genes of the differentially expressed lncRNAs (DElncRNAs) in sperm freezability. We detected approximately 4007 DElncRNAs, which were differentially expressed in spermatozoa from boars classified as having good and poor semen freezability (GSF and PSF, respectively). Most of the DElncRNAs were upregulated in boars of the PSF group and appeared to significantly affect the sperm's response to the cryopreservation conditions. Furthermore, we predicted that the potential target genes were regulated by DElncRNAs in or . It was found that DElncRNAs of both freezability groups had potential - and -regulatory effects on different protein-coding genes, such as , and . Gene Ontology (GO) enrichment revealed that the DElncRNA target genes are associated with numerous biological processes, including signal transduction, response to stress, cell death (apoptosis), motility and embryo development. Significant differences in the assembled transcriptome expression profiles of the DElncRNAs between the freezability groups were confirmed by quantitative real-time PCR analysis. This study reveals the potential effects of protein-coding genes of DElncRNAs on sperm functions, which could contribute to further research on their relevance in semen freezability.
长链非编码RNA（lncRNAs）被认为在精子生物学过程中发挥重要作用。我们进行了转录组组装，以表征精子中的lncRNAs，并研究差异表达lncRNAs（DElncRNAs）的潜在靶基因在精子冷冻保存能力中的作用。我们检测到约4007个DElncRNAs，它们在分别被归类为具有良好和较差精液冷冻保存能力（分别为GSF和PSF）的公猪精子中差异表达。大多数DElncRNAs在PSF组公猪中上调，并且似乎显著影响精子对冷冻保存条件的反应。此外，我们预测潜在靶基因在或中受DElncRNAs调控。结果发现，两个冷冻保存能力组的DElncRNAs对不同的蛋白质编码基因具有潜在的和调控作用，如、和。基因本体论（GO）富集分析表明，DElncRNA靶基因与众多生物学过程相关，包括信号转导、应激反应、细胞死亡（凋亡）、运动性和胚胎发育。通过定量实时PCR分析证实了冷冻保存能力组之间DElncRNAs组装转录组表达谱的显著差异。本研究揭示了DElncRNAs的蛋白质编码基因对精子功能的潜在影响，这可能有助于进一步研究它们在精液冷冻保存能力中的相关性。
