Redpath J L, Sun C, Colman M, Stanbridge E J
Radiat Res. 1987 Jun;110(3):468-72.
Human skin fibroblasts are extremely refractory to neoplastic transformation by ionizing radiation [C. Borek, Nature 283, 776-778 (1980); M. Namba, H. Nishitani, and T. Kimoto, J. Exp. Med. 48, 303-311 (1978)] and are therefore unsuitable for quantitative studies of dose-effect relationships. We show here that a nontumorigenic human hybrid cell line (HeLa X skin fibroblast) can be neoplastically transformed by treatment with gamma radiation. Furthermore, a dose-response relationship has been established. We propose that this human hybrid cell line may be a useful system for mechanistic studies of transformation from the preneoplastic to the neoplastic state by ionizing radiation and other agents.
人类皮肤成纤维细胞对电离辐射诱导的肿瘤转化具有极强的抗性[C. 博雷克，《自然》283, 776 - 778 (1980)；M. 南波、H. 西谷和T. 木本，《实验医学杂志》48, 303 - 311 (1978)]，因此不适合用于剂量 - 效应关系的定量研究。我们在此表明，一种非致瘤性人类杂交细胞系（HeLa×皮肤成纤维细胞）经γ射线处理后可发生肿瘤转化。此外，还建立了剂量 - 反应关系。我们认为，这种人类杂交细胞系可能是用于研究电离辐射及其他因素诱导从癌前状态向肿瘤状态转化机制的有用系统。
