Chrapusta-Srebrny Ewelina, Bialczyk Jan, Duchnik Kornelia, Bober Beata
Department of Plant Physiology and Development, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Department of Microbiology, Institute of Quality Sciences and Product Management, Cracow University of Economics, Rakowicka 27, 31-510 Krakow, Poland.
Metabolites. 2022 Jul 10;12(7):632. doi: 10.3390/metabo12070632.
in its environmental niches is regularly affected by daily and annual variations in solar radiation. Mycosporine-glutamicol, Myc-Glu(OH), which it synthesizes, may act as a significant cellular UV-protector. Therefore, we studied this compound concentration in lichen thalli concerning seasonal changes and increased exposure to UV-B and photosynthetically active radiation (PAR) with/without simultaneous CO deprivation. Myc-Glu(OH) occurred year-round and exhibited a strong seasonality. The most crucial role in the control of its synthesis played UV-B radiation, although its high concentration was also found after PAR irradiation at 1000 µmol m s. As PAR intensity increased to 2000 µmol m s, the rate of Myc-Glu(OH) synthesis slowed down. In turn, under dark/PAR irradiation with simultaneous deprivation of CO in the atmosphere surrounding and during darkness with continuous access to atmospheric CO, its production was insignificant. Obtained data confirmed that Myc-Glu(OH) plays an important role in protecting from UV damage and favours its adaptation to environmental stress in its natural habitat. They also suggest that its synthesis is a synergism of multiple factors. Consequently, further studies should focus on their evaluation and the identification of a lichen partner actively involved in Myc-Glu(OH) biogenesis.
在其生态位中，它经常受到太阳辐射的日变化和年变化的影响。它合成的肌醇六磷酸 - 谷氨酸醇（Myc-Glu(OH)）可能作为一种重要的细胞紫外线保护剂。因此，我们研究了地衣叶状体中这种化合物的浓度与季节变化以及在有/无同时二氧化碳剥夺的情况下增加紫外线B和光合有效辐射（PAR）暴露之间的关系。Myc-Glu(OH)全年都有，并且表现出强烈的季节性。在其合成控制中起最关键作用的是紫外线B辐射，尽管在1000 μmol m⁻² s⁻¹的PAR照射后也发现其浓度很高。随着PAR强度增加到2000 μmol m⁻² s⁻¹，Myc-Glu(OH)的合成速率减慢。反过来，在黑暗/PAR照射以及周围大气中同时剥夺二氧化碳的情况下，以及在黑暗中持续接触大气二氧化碳的情况下，其产量微不足道。获得的数据证实，Myc-Glu(OH)在保护其免受紫外线损伤方面发挥着重要作用，并有利于其在自然栖息地适应环境压力。它们还表明其合成是多种因素的协同作用。因此，进一步的研究应侧重于对这些因素的评估以及鉴定积极参与Myc-Glu(OH)生物合成的地衣共生伙伴。
