Xu Shanshan, Cao Qianqian, Liu Zengzhi, Chen Junpeng, Yan Peiguang, Li Bingyu, Xu Ying
Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518055, China.
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Microorganisms. 2022 Jul 1;10(7):1338. doi: 10.3390/microorganisms10071338.
strains are widely distributed in terrestrial and marine environments, and some of them are used as biocontrol organisms for their biofilm-formation ability. In , biofilm formation is fine-tuned by a complex network, a clear understanding of which still requires study. In bacteria, tmRNA, encoded by the gene, catalyzes -translation that can rescue ribosomes stalled on mRNA transcripts lacking a functional stop codon. tmRNA also affects physiological bioprocesses in some bacteria. In this study, we constructed a mutant in and found that the biofilm formation in the mutant was largely impaired. Moreover, we isolated a biofilm-formation suppressor of , in which the biofilm formation was restored to a level even stronger than that in the wild type. We further performed RNAseq assays with the wild type, mutant, and suppressor of for comparisons of their transcriptomes. By analyzing the transcriptomic data, we predicted the possible functions of some differentially expressed genes (DEGs) in the tmRNA regulation of biofilm formation in . Finally, we found that the overexpression of two DEGs, and , could restore the biofilm formation in the mutant, indicating that AcoA and YhjR were immediate regulators involved in the tmRNA regulatory web controlling biofilm formation in . Our data can improve the knowledge about the molecular network involved in biofilm formation and provide new targets for manipulation of biofilms for future investigation.
菌株广泛分布于陆地和海洋环境中，其中一些因其生物膜形成能力而被用作生物防治生物。在[具体研究对象]中，生物膜的形成由一个复杂的网络进行微调，对其的清晰理解仍需深入研究。在细菌中，由[具体基因]编码的tmRNA催化[具体反应]，该反应可拯救停滞在缺乏功能性终止密码子的mRNA转录本上的核糖体。tmRNA还影响一些细菌的生理生物过程。在本研究中，我们构建了[具体菌株]的[具体基因]突变体，发现该突变体中的生物膜形成受到很大损害。此外，我们分离出了[具体菌株]的生物膜形成抑制因子，其中生物膜形成恢复到甚至比野生型更强的水平。我们进一步对野生型、[具体基因]突变体和[具体菌株]的抑制因子进行了RNAseq分析，以比较它们的转录组。通过分析转录组数据，我们预测了一些差异表达基因（DEGs）在[具体菌株]生物膜形成的tmRNA调控中的可能功能。最后，我们发现两个DEGs，[具体基因1]和[具体基因2]的过表达可以恢复[具体基因]突变体中的生物膜形成，这表明AcoA和YhjR是参与[具体菌株]生物膜形成的tmRNA调控网络的直接调节因子。我们的数据可以增进对[具体菌株]生物膜形成所涉及的分子网络的了解，并为未来研究中操纵[具体菌株]生物膜提供新的靶点。
