Xu Hao, Cai Qingtao, An Qiuying, Tang Chen, Wang Wanpeng, Wang Guangshun, You Wanting, Guo Dongbei, Zhao Ran
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China.
The Second People's Hospital of Futian District in Shenzhen, Shenzhen 518000, China.
Microorganisms. 2022 Jul 17;10(7):1441. doi: 10.3390/microorganisms10071441.
Decabromodiphenyl ether (BDE-209), a polybrominated diphenyl ether (PBDE) homolog, seriously threatens human health. In this study, a strain with high BDE-209 degradation activity, named TAW-CT127, was isolated from Tong'an Bay, Xiamen. Under laboratory conditions, the strain's optimal growth temperature, pH, and salinity are 45 °C, 7.0, and 0-2.5%, respectively. Scanning electron microscopy (SEM) analysis shows that TAW-CT127 is damaged when grown in manual marine culture (MMC) medium with BDE-209 as the sole carbon source instead of eutrophic conditions. In the dark, under the conditions of 28 °C, 160 rpm, and 3 g/L (wet weight) TAW-CT127, the degradation rate of 50 mg/L BDE-209 is 81.07%. The intermediate metabolites are hexabromo-, octabromo-, and nonabromo-diphenyl ethers. Through whole-genome sequencing, multiple dehalogenases were found in the genome of TAW-CT127; these may be involved in the production of lower-brominated diphenyl ethers. Additionally, biphenyl-2,3-dioxygenase (BDO) in TAW-CT127 may catalyze the debromination reaction of BDE-209. Our research provides a new high-efficiency strain for bioremediation of BDE-209 pollution, and lays the foundation for the preliminary exploration of genes associated with BDE-209 degradation.
十溴二苯醚（BDE-209）是一种多溴二苯醚（PBDE）同系物，严重威胁人类健康。在本研究中，从厦门同安湾分离出一株具有高BDE-209降解活性的菌株，命名为TAW-CT127。在实验室条件下，该菌株的最佳生长温度、pH值和盐度分别为45℃、7.0和0-2.5%。扫描电子显微镜（SEM）分析表明，当TAW-CT127在以BDE-209为唯一碳源的人工海水培养基（MMC）中生长而非富营养条件下时会受到损伤。在黑暗中，于28℃、160 rpm和3 g/L（湿重）TAW-CT127的条件下，50 mg/L BDE-209的降解率为81.07%。中间代谢产物为六溴、八溴和九溴二苯醚。通过全基因组测序，在TAW-CT127的基因组中发现了多种脱卤酶；这些酶可能参与了低溴化二苯醚的生成。此外，TAW-CT127中的联苯-2,3-双加氧酶（BDO）可能催化BDE-209的脱溴反应。我们的研究为BDE-209污染的生物修复提供了一种新的高效菌株，并为与BDE-209降解相关基因的初步探索奠定了基础。
