Department of Chemistry, Kulliyyah of Science, International Islamic University Malaysia, Kuantan 25200, Malaysia.
Sustainable Chemistry Research Group (SusChemRG), Kulliyyah of Science, International Islamic University Malaysia, Kuantan 25200, Malaysia.
Molecules. 2022 Jul 12;27(14):4459. doi: 10.3390/molecules27144459.
Vitamin B12, only found naturally in animal-based foods, is essential for brain functions and various chemical reactions in the human body. Insufficient vitamin B12 leads to vitamin B12 deficiency, common among strict vegetarians due to their limited intake of animal-based foods. Nevertheless, extensive studies have demonstrated that macroalgae, specifically the species, are rich in vitamin B12 and could be further exploited in future dietary applications. In the current study, the ideal extraction method of vitamin B12 from dried was developed and optimised to achieve the maximum vitamin B12 yield. The effects of several extraction parameters, including the solvent-to-solvent, methanol:water (MeOH:HO), and solute-to-solvent ratios, and pH on the total vitamin B12 content were analysed through a two-level factorial and central composite design. The highest vitamin B12 content, particularly cyanocobalamin (CN-Cbl), was recovered through the ultrasonic-assisted extraction (UAE) of oven-dried at 3 g:60 mL of solute-to-solvent and 25:75% of MeOH to HO ratios at pH 4. The extraction of CN-Cbl from oven-dried that employed the UAE method has elevated CN-Cbl content recovery compared to other extraction methods.
维生素 B12 仅存在于动物源性食物中，是大脑功能和人体各种化学反应所必需的。维生素 B12 摄入不足会导致维生素 B12 缺乏，严格的素食者由于其动物源性食物摄入量有限，因此常见维生素 B12 缺乏。然而，大量研究表明，大型藻类，特别是 物种，富含维生素 B12，可以在未来的饮食应用中进一步开发。在本研究中，开发并优化了从干燥 中提取维生素 B12 的理想方法，以获得最大的维生素 B12 产量。通过两水平因子和中心复合设计分析了几种提取参数（包括溶剂比、甲醇：水（MeOH：HO）和固液比以及 pH 值）对总维生素 B12 含量的影响。通过在 pH 值为 4 时，用超声辅助提取（UAE）法，从 3 g：60 mL 的固液比和 25：75%的 MeOH 到 HO 比例的干燥 中提取，获得了最高的维生素 B12 含量，特别是氰钴胺素（CN-Cbl）。与其他提取方法相比，UAE 法从干燥 中提取 CN-Cbl 可提高 CN-Cbl 的含量回收率。
