Guo Ningyuan, Liu Jingwen, He Qing, Zhou Rongyan, Yuan Haobo
School of Electrical Engineering and Telecommunications, School of Engineering, University of New South Wales, Library Rd, Kensington, NSW 2033, Australia.
UniSA STEM, University of South Australia, Mawson Lakes Blvd, Salisbury, SA 5095, Australia.
Biomimetics (Basel). 2022 Jul 9;7(3):94. doi: 10.3390/biomimetics7030094.
A fluorescence device based on ultraviolet light is proposed in this paper, which currently stands at the design stage with the eventual aim to potentially detect virus/antibody fluorescence reactions. The designed device is proposed to have the characteristics of high reflectivity, low power consumption, wide spectrum of light source, and proper silver coating. For fabrication and raising product quality, 3D printing technology and a sputtering test will be used. In this connection, this paper firstly introduces the design sources; then, the ideas of inventing fluorescence detection devices based on ultraviolet light, followed by the data analysis as well as discussing the results of computer simulations. The design process, materials, methods, and experiments are demonstrated following the reality work procedure. Instead of directly using a virus or antibodies for the experiment, at the current design stage, we focus on using this device to detect the rhodamine B reagent. Experiment shows that this reagent can be successfully detected. With this achievement, we logically believe that such type of an ultraviolet optical sensor, with further development and testing, may have the possible value to detect a single virus such as COVID-19, as well as other viruses or small molecules. Though there is long way to go to achieve such a goal, future works experimenting with the detection device on real virus or antibodies can take place more efficiently with a good foundation.
本文提出了一种基于紫外光的荧光检测装置，目前该装置处于设计阶段，最终目标是潜在地检测病毒/抗体荧光反应。所设计的装置具有高反射率、低功耗、宽光谱光源以及合适的银涂层等特性。为了进行制造并提高产品质量，将使用3D打印技术和溅射测试。在此方面，本文首先介绍设计来源；然后，阐述基于紫外光发明荧光检测装置的思路，接着进行数据分析并讨论计算机模拟结果。按照实际工作流程展示设计过程、材料、方法和实验。在当前设计阶段，并非直接使用病毒或抗体进行实验，而是专注于使用该装置检测罗丹明B试剂。实验表明该试剂能够被成功检测。基于这一成果，我们合理地认为，这种紫外光学传感器经过进一步开发和测试，可能具有检测诸如新冠病毒等单一病毒以及其他病毒或小分子的潜在价值。尽管要实现这一目标还有很长的路要走，但有了良好的基础，未来使用该检测装置对真实病毒或抗体进行实验的工作可以更高效地开展。
