Institute of Food Safety and Risk Management, National Taiwan Ocean University, Keelung City 202, Taiwan.
Department of Food Science, National Taiwan Ocean University, Keelung City 202, Taiwan.
Biosensors (Basel). 2022 Jul 25;12(8):561. doi: 10.3390/bios12080561.
The label-free biosensor has emerged as an effective tool for the purpose of early detection of causative pathogens such as as a preventive measure. In this study, a biorecognition-element-free interdigitated microelectrode (IDμE) sensor is designed and developed with this in mind, with good reliability and affordability. Results show that the designed sensor can identify with good selectivity using an impedance and capacitance of 7.69 MHz. At its optimum impedance of 1.3 kHz, the IDμE sensor can reliably quantify in a range of measurement (10~10 cfu/mL), linearity (R = 0.97), sensitivity (18.15 kΩ/log (cfu/mL)), and limit of detection (10 cfu/mL). In summary, the IDμE sensor developed possesses high potential for industrial and clinical applications.
无标记生物传感器已成为早期检测病原体（如 ）等致病病原体的有效工具，作为预防措施。在这项研究中，设计并开发了一种无需生物识别元件的叉指微电极（IDμE）传感器，具有良好的可靠性和可负担性。结果表明，所设计的传感器可以使用 7.69 MHz 的阻抗和电容来识别 ，具有良好的选择性。在最佳阻抗为 1.3 kHz 时，IDμE 传感器可以可靠地在测量范围内（10~10 cfu/mL）定量 ，线性度（R = 0.97），灵敏度（18.15 kΩ/log（cfu/mL））和检测限（10 cfu/mL）。综上所述，所开发的 IDμE 传感器具有很高的工业和临床应用潜力。
