Liang Ying, Wang Lin, Zhang Ruipeng, Pan Jiadi, Wu Wenhong, Huang Yuanyuan, Zhang Zifan, Zhao Renbang
Faculty of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China.
Animals (Basel). 2022 Jul 23;12(15):1885. doi: 10.3390/ani12151885.
This paper developed a universal detection method by high-performance liquid chromatography-tandem mass spectrometry to detect three typical clenbuterols, CLB, SAL, and RAC, and to investigate the metabolism of β-agonists in vivo. The parent ions and daughter ions of the three β-receptor agonist standards and the residues in the muscle, liver, and blood samples of rats were obtained by Total Ions Scan mode. The metabolites produced in different tissues at a specific time were qualitatively and quantitatively analyzed, and the corresponding metabolic pathways were inferred. The results showed that the three β-receptor agonists mainly existed in the form of prototype drugs in rats, with a small amount of clenbuterol methyl compound and albuterol methyl compound. There were significant differences in residual metabolism between different tissues of the same species. In addition, different β-receptor agonists have different absorption and utilization rates in rats.
本文开发了一种通过高效液相色谱-串联质谱法的通用检测方法，以检测三种典型的克伦特罗、沙丁胺醇和莱克多巴胺，并研究β-激动剂在体内的代谢情况。通过全离子扫描模式获得了三种β受体激动剂标准品以及大鼠肌肉、肝脏和血液样本中残留物的母离子和子离子。对特定时间不同组织中产生的代谢物进行了定性和定量分析，并推断了相应的代谢途径。结果表明，三种β受体激动剂在大鼠体内主要以原型药物形式存在，有少量克伦特罗甲基化合物和沙丁胺醇甲基化合物。同一物种不同组织之间的残留代谢存在显著差异。此外，不同的β受体激动剂在大鼠体内有不同的吸收和利用率。
