Guzzeloni Virginia, Veschini Lorenzo, Pedica Federica, Ferrero Elisabetta, Ferrarini Marina
B-Cell Neoplasia Unit, Division of Experimental Oncology, IRCCS Ospedale San Raffaele, 20132 Milan, Italy.
Academic Centre of Reconstructive Science, Faculty of Dentistry Oral & Craniofacial Sciences, King's College London, Guy's Hospital, London SE1 9RT, UK.
Antibodies (Basel). 2022 Jul 8;11(3):46. doi: 10.3390/antib11030046.
Therapeutic monoclonal antibodies (mAbs) are an emerging and very active frontier in clinical oncology, with hundred molecules currently in use or being tested. These treatments have already revolutionized clinical outcomes in both solid and hematological malignancies. However, identifying patients who are most likely to benefit from mAbs treatment is currently challenging and limiting the impact of such therapies. To overcome this issue, and to fulfill the expectations of mAbs therapies, it is urgently required to develop proper culture models capable of faithfully reproducing the interactions between tumor and its surrounding native microenvironment (TME). Three-dimensional (3D) models which allow the assessment of the impact of drugs on tumors within its TME in a patient-specific context are promising avenues to progressively fill the gap between conventional 2D cultures and animal models, substantially contributing to the achievement of personalized medicine. This review aims to give a brief overview of the currently available 3D models, together with their specific exploitation for therapeutic mAbs testing, underlying advantages and current limitations to a broader use in preclinical oncology.
治疗性单克隆抗体（mAbs）是临床肿瘤学中一个新兴且非常活跃的前沿领域，目前有数百种分子正在使用或处于试验阶段。这些治疗方法已经彻底改变了实体瘤和血液系统恶性肿瘤的临床治疗效果。然而，目前确定最有可能从单克隆抗体治疗中获益的患者具有挑战性，这限制了此类疗法的影响。为了克服这个问题，并实现单克隆抗体疗法的期望，迫切需要开发能够忠实地再现肿瘤与其周围天然微环境（TME）之间相互作用的合适培养模型。三维（3D）模型能够在患者特异性背景下评估药物对肿瘤及其TME的影响，是逐步填补传统二维培养和动物模型之间差距的有前景的途径，对实现个性化医疗有很大贡献。本综述旨在简要概述当前可用的3D模型，以及它们在治疗性单克隆抗体测试中的具体应用、潜在优势和在临床前肿瘤学中更广泛应用的当前局限性。
