Laboratory of Molecular Immunology, Federal State Budgetary Scientific Institution Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, Novosibirsk 630099, Russia.
Clinic of Immunopathology, Federal State Budgetary Scientific Institution Research Institute of Fundamental and Clinical Immunology, Zalesskogo 2/1, Novosibirsk 630047, Russia.
Genes (Basel). 2022 Jul 26;13(8):1333. doi: 10.3390/genes13081333.
CD71+ erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchymaCECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchymaCECs only. These observations suggest that there might be undiscovered roles in immune response for CECs.
CD71+ 红系细胞（CECs）直到不久前才被认为是红细胞的祖细胞。然而，现在已经证明它们是免疫调节的积极参与者，特别是通过 ROS、精氨酸酶-1 和精氨酸酶-2 产生来进行免疫抑制。在这里，我们使用 NanoString 揭示了健康成人骨髓和人胎肝实质来源的 CECs 之间细胞因子基因表达的器官起源差异，并使用 Bio-Plex 检测了细胞因子蛋白的产生。具体来说，健康成人骨髓 CECs 表达和产生 IFN-a、IL-1b、IL-8、IL-18 和 MIF mRNA 和蛋白，而人胎肝实质 CECs 表达和产生 IFN-a、IL15、IL18 和 TNF-b mRNA 和蛋白。我们还检测到两种 CECs 中 TLR2 和 TLR9 的基因表达，以及人胎肝实质 CECs 中 TLR1 和 NOD2 的基因表达。这些观察结果表明，CECs 在免疫反应中可能有尚未发现的作用。
