Chen Jie, Li Zhi, Sheng Xiaotian, Huang Jun, Sun Quan, Huang Yukang, Wang Rong, Wu Yujiao, Long Mengxian, Bao Jialing, Zhou Zeyang, Pan Guoqing
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.
Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400716, China.
J Fungi (Basel). 2022 Jul 23;8(8):764. doi: 10.3390/jof8080764.
Microsporidia are a big group of single-celled obligate intracellular organisms infecting most animals and some protozoans. These minimalist eukaryotes lack numerous genes in metabolism and vesicle trafficking. Here, we demonstrated that the spore wall protein NbSWP12 of microsporidium belongs to Bin/Amphiphysin/Rvs (BAR) protein family and can specifically bind with phosphatidylinositol 3-phosphate [Ptdlns(3)P]. Since Ptdlns(3)P is involved in endosomal vesicle biogenesis and trafficking, we heterologous expressed NbSWP12 in yeast and proved that NbSWP12 can target the cell membrane and endocytic vesicles. transformed into (a BAR protein of ) deletion mutant rescued the defect phenotype of vesicular traffic. This study identified a BAR protein function in vesicle genesis and sorting and provided clues for further understanding of how microsporidia internalize nutrients and metabolites during proliferation.
微孢子虫是一大类单细胞专性细胞内生物，可感染大多数动物和一些原生动物。这些极简真核生物在代谢和囊泡运输方面缺乏众多基因。在此，我们证明微孢子虫的孢子壁蛋白NbSWP12属于Bin/Amphiphysin/Rvs（BAR）蛋白家族，并且能够特异性结合磷脂酰肌醇3-磷酸[Ptdlns(3)P]。由于Ptdlns(3)P参与内体囊泡生物发生和运输，我们在酵母中异源表达NbSWP12，并证明NbSWP12可靶向细胞膜和内吞囊泡。转化到（一种的BAR蛋白）缺失突变体中可挽救囊泡运输的缺陷表型。本研究确定了BAR蛋白在囊泡发生和分选中的功能，并为进一步了解微孢子虫在增殖过程中如何内化营养物质和代谢产物提供了线索。
