Reichelt-Wurm Simone, Pregler Matthias, Wirtz Tobias, Kretz Markus, Holler Kathrin, Banas Bernhard, Banas Miriam C
Department of Nephrology, University Hospital Regensburg, 93053 Regensburg, Germany.
Regensburg Center for Biochemistry (RCB), University of Regensburg, 93053 Regensburg, Germany.
Noncoding RNA. 2022 Jul 13;8(4):52. doi: 10.3390/ncrna8040052.
Mesangial cells (MCs), substantial cells for architecture and function of the glomerular tuft, take a key role in progression of diabetic kidney disease (DKD). Despite long standing researches and the need for novel therapies, the underlying regulatory mechanisms in MCs are elusive. This applies in particular to long non-coding RNAs (lncRNA) but also microRNAs (miRNAs). In this study, we investigated the expression of nuclear paraspeckle assembly transcript 1 (), a highly conserved lncRNA, in several diabetes in-vitro models using human MCs. These cells were treated with high glucose, TGFβ, TNAα, thapsigargin, or tunicamycin. We analyzed the implication of silencing on mesangial cell migration, proliferation, and cell size as well as on mRNA and miRNA expression. Here, the miRNA hsa-miR-339-5p was not only identified as a potential interaction partner for but also for several coding genes. Furthermore, overexpression of hsa-miR-339-5p leads to a MC phenotype comparable to a knockdown. In-silico analyses also underline a relevant role of and hsa-miR-339-5p in mesangial physiology, especially in the context of DKD.
系膜细胞（MCs）是肾小球结构和功能的重要细胞，在糖尿病肾病（DKD）进展中起关键作用。尽管进行了长期研究且需要新的治疗方法，但MCs中的潜在调控机制仍不清楚。这尤其适用于长链非编码RNA（lncRNA），但也适用于微小RNA（miRNA）。在本研究中，我们使用人MCs在几种糖尿病体外模型中研究了高度保守的lncRNA核旁斑组装转录本1（）的表达。这些细胞用高糖、转化生长因子β、肿瘤坏死因子α、毒胡萝卜素或衣霉素处理。我们分析了沉默对系膜细胞迁移、增殖、细胞大小以及mRNA和miRNA表达的影响。在此，miRNA hsa-miR-339-5p不仅被鉴定为与的潜在相互作用伙伴，而且还与几个编码基因相互作用。此外，hsa-miR-339-5p的过表达导致与敲低相当的MC表型。计算机分析也强调了和hsa-miR-339-5p在系膜生理学中的相关作用，特别是在DKD背景下。
