用聚合酶链式反应方法检测波兰西南部患牛呼吸道疾病奶牛的七种病原体的流行情况、共存情况及相关性

The Prevalence, Coexistence, and Correlations between Seven Pathogens Detected by a PCR Method from South-Western Poland Dairy Cattle Suffering from Bovine Respiratory Disease.

作者信息

Lachowicz-Wolak Agnieszka, Klimowicz-Bodys Małgorzata D, Płoneczka-Janeczko Katarzyna, Bykowy Marek, Siedlecka Magdalena, Cinciała Jagoda, Rypuła Krzysztof

机构信息

Division of Infectious Diseases of Animals and Veterinary Administration, Department of Epizootiology and Clinic of Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.

Student Scientific Society "AnthraX", Department of Epizootiology and Clinic of Birds and Exotic Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wroclaw, Poland.

出版信息

Microorganisms. 2022 Jul 24;10(8):1487. doi: 10.3390/microorganisms10081487.

Bovine respiratory disease (BRD) is a very important disease that contributes to economic losses in dairy and beef cattle breeding worldwide. The molecular testing of material from 296 calves showing BRD symptoms from 74 dairy herds located in south-western Poland was performed in 2019-2021. Molecular tests were performed using a commercial kit "VetMAX Ruminant Respiratory Screening Kit" (Thermo Fisher Scientific) for the simultaneous detection of genetic material of seven pathogens responsible for BRD. At least one pathogen was detected in 95.95% of herds. The overall prevalence was: 87.84%, 44.59%, bovine coronavirus (BcoV) 32.43%, 29.73%, 28.38%, bovine parainfluenza virus type 3 (BPIV-3) 13.51%, and bovine respiratory syncytial virus (BRSV) 10.81%. Twenty-nine configurations of pathogen occurrences were found. Bacterial infections were the most frequently recorded as 56.7% of all results. Coinfections mainly consisted of two pathogens. Not a single purely viral coinfection was detected. The most frequent result was a single infection accounting for 18.31% of all results. The statistically significant correlation ( = 0.001) with the highest strength of effect (ϕ 0.38) was between and .

牛呼吸道疾病（BRD）是一种非常重要的疾病，在全球奶牛和肉牛养殖中造成经济损失。2019年至2021年，对波兰西南部74个奶牛场中表现出BRD症状的296头犊牛的材料进行了分子检测。使用商业试剂盒“VetMAX反刍动物呼吸道筛查试剂盒”（赛默飞世尔科技）进行分子检测，以同时检测导致BRD的七种病原体的遗传物质。在95.95%的牛群中检测到至少一种病原体。总体患病率为：87.84%，44.59%，牛冠状病毒（BcoV）32.43%，29.73%，28.38%，牛副流感病毒3型（BPIV-3）13.51%，牛呼吸道合胞病毒（BRSV）10.81%。发现了29种病原体出现情况的组合。细菌感染是最常记录到的，占所有结果的56.7%。混合感染主要由两种病原体组成。未检测到单一的纯病毒混合感染。最常见的结果是单一感染，占所有结果的18.31%。与最高效应强度（ϕ 0.38）具有统计学显著相关性（=0.001）的是和之间。