突尼斯首次报告两种啮齿动物可能是寄生虫的储存宿主。

First Report of Two Rodents as Potential Reservoir Hosts of Parasites in Tunisia.

作者信息

Ghawar Wissem, Chaouch Melek, Ben Salah Afif, Snoussi Mohamed Ali, Salem Sadok, Kharroubi Ghassen, Chouchen Said, Bouaoun Amor, Laouini Dhafer, Bettaieb Jihene, Ben Abderrazak Souha

机构信息

Department of Medical Epidemiology, Institut Pasteur de Tunis, Tunis 1002, Tunisia.

Laboratory of Transmission, Control and Immunobiology of Infections (LR16IPT02), Institut Pasteur de Tunis, Tunis 1002, Tunisia.

出版信息

Microorganisms. 2022 Jul 25;10(8):1502. doi: 10.3390/microorganisms10081502.

This study shows, for the first time, natural infection among spp. in an endemic region of Tataouine, South Tunisia. To better characterize the transmission cycles in this complex focus of mixed transmission, detection and species identification were performed by direct examination, internal transcribed spacer-1 (ITS1)-PCR-restriction fragment length polymorphism (RFLP), and sequencing of (.) (Linnaeus, 1758) and . (Lichtenstein, 1823) rodent species, which are frequently encountered in this area. parasites were observed in 19 (41.3%) smears, while DNA parasites were detected in 28 (60.9%) spp. spleens; among them, 12 (54.5%) were from 22 . individuals and 16 (66.7%) were from 24 . individuals. parasites were confirmed as (.) (syn. . ) in two . individuals (4.3%) and . (n = 24; 52.2%) in 10 and 14 individuals. This finding represents the first evidence of natural infection with parasites in rodents belonging to the genus, providing the rationale to consider them as potential reservoir hosts of Old World parasites in Tunisia and North Africa.

本研究首次表明，在突尼斯南部塔塔温的一个流行地区，[具体物种名称]属物种中存在自然感染情况。为了更好地描述这个混合传播的复杂疫源地中的传播循环，通过直接检查、内转录间隔区1（ITS1）-聚合酶链反应-限制性片段长度多态性（RFLP）以及对该地区常见的[具体物种名称1]（Linnaeus，1758）和[具体物种名称2]（Lichtenstein，1823）啮齿动物物种进行测序来进行检测和物种鉴定。在19份涂片（41.3%）中观察到了寄生虫，而在28份[具体物种名称]属脾脏样本（60.9%）中检测到了寄生虫DNA；其中，12份（54.5%）来自22只[具体物种名称1]个体，16份（66.7%）来自24只[具体物种名称2]个体。在两名[具体物种名称1]个体（4.3%）中确认寄生虫为[具体寄生虫名称1]（同物异名[具体名称]），在10只和14只[具体物种名称2]个体中确认寄生虫为[具体寄生虫名称2]（n = 24；52.2%）。这一发现代表了属于[具体属名]属的啮齿动物中自然感染[具体寄生虫名称]寄生虫的首个证据，为将它们视为突尼斯和北非旧世界[具体寄生虫名称]寄生虫的潜在储存宿主提供了理论依据。