State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, China.
Toxins (Basel). 2022 Jul 24;14(8):507. doi: 10.3390/toxins14080507.
To control the fall armyworm (FAW), (Smith), a serious threat to maize production in China, the Chinese government has issued biosafety certificates for transgenic insect-resistant maize expressing Bt () toxins including Bt-Cry1Ab maize (crop event DBN9936), Bt-Vip3Aa maize (event DBN9501), Bt-(Cry1Ab+Vip3Aa) maize with superimposed traits (event DBN9936 × DBN9501) and Bt-(Cry1Ab+Vip3Aa) maize with superimposed traits (event Bt11 × MIR162), but the susceptibility baselines of geographically distinct FAW populations to these events, which form the basis for managing resistance development in the pest to these events, are not clear. We used the diet-incorporated bioassays method to detect the susceptibilities of the seven FAW populations collected from Yunnan, Henan and Hubei provinces in China in 2021 to the insecticidal proteins of the four Bt maize events. The result showed that the susceptibilities of different geographical populations to Bt insecticidal proteins were significantly different. In the seven populations, the range in median lethal concentrations (LC) of Cry1Ab expressed in DBN9936 was 0.87-2.63 μg/g, 0.14-0.30 μg/g for Vip3Aa expressed in DBN9501, 0.78-1.86 μg/g for Cry1Ab+Vip3Aa expressed in DBN9936 × DBN9501, and 0.36-1.42 μg/g for CryAb+Vip3Aa expressed in Bt11 × MIR162. The growth inhibition responses also showed that the susceptibilities varied with the different median growth inhibitory concentration (GIC) ranges (0.38-1.22, 0.08-0.28, 0.28-0.87, and 0.24-0.78 μg/g, respectively). The variations in the ranges of the susceptibility baselines of the geographical populations of fall armyworm in China to the insecticidal proteins expressed in the four events provide a scientific basis for monitoring FAW population resistance to Bt maize and managing the populations using different Bt maize events.
为了控制草地贪夜蛾（FAW），中国政府已为表达 Bt 毒素的抗虫转基因玉米颁发了生物安全证书，包括表达 Cry1Ab 毒素的 Bt-Cry1Ab 玉米（事件 DBN9936）、表达 Vip3Aa 毒素的 Bt-Vip3Aa 玉米（事件 DBN9501）、具有叠加特性的 Bt-(Cry1Ab+Vip3Aa)玉米（事件 DBN9936×DBN9501）和具有叠加特性的 Bt-(Cry1Ab+Vip3Aa)玉米（事件 Bt11×MIR162），这些玉米对玉米生产构成了严重威胁。然而，这些事件所形成的地理上不同的 FAW 种群对这些事件的敏感性基线尚不清楚，而这些敏感性基线是管理害虫对这些事件产生抗药性的基础。我们使用了饲料掺入生物测定法来检测 2021 年在中国云南、河南和湖北采集的 7 个 FAW 种群对这 4 种 Bt 玉米事件的杀虫蛋白的敏感性。结果表明，不同地理种群对 Bt 杀虫蛋白的敏感性存在显著差异。在这 7 个种群中，DBN9936 中表达的 Cry1Ab 的中致死浓度（LC）范围为 0.87-2.63μg/g，DBN9501 中表达的 Vip3Aa 的 LC 范围为 0.14-0.30μg/g，DBN9936×DBN9501 中表达的 Cry1Ab+Vip3Aa 的 LC 范围为 0.78-1.86μg/g，Bt11×MIR162 中表达的 Cry1Ab+Vip3Aa 的 LC 范围为 0.36-1.42μg/g。生长抑制反应也表明，敏感性随不同的中生长抑制浓度（GIC）范围（0.38-1.22、0.08-0.28、0.28-0.87 和 0.24-0.78μg/g）而变化。中国 FAW 地理种群对这 4 种事件表达的杀虫蛋白的敏感性基线范围的变化为监测 FAW 种群对 Bt 玉米的抗性和使用不同的 Bt 玉米事件管理种群提供了科学依据。
