Puah Suat Moi, Khor Wei Ching, Aung Kyaw Thu, Lau Tien Tien Vicky, Puthucheary S D, Chua Kek Heng
Department of Biomedical Science, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
National Centre for Food Science, Singapore Food Agency, 52 Jurong Gateway Road, JEM Office Tower, 14-01, Singapore 608550, Singapore.
Pathogens. 2022 Jul 26;11(8):833. doi: 10.3390/pathogens11080833.
is ubiquitous in aquatic habitats and can cause life-threatening septicaemia in humans. However, limited data are available on their antimicrobial susceptibility testing (AST) profiles. Hence, we aimed to examine their AST patterns using clinical ( = 94) and non-clinical ( = 23) isolates with dehydrated MicroScan microdilution. Carbapenem resistant isolates were further screened for genes related to carbapenem resistance using molecular assay. The isolates exhibited resistance to imipenem (76.9%), doripenem (62.4%), meropenem (41.9%), trimethoprim/sulfamethoxazole (11.1%), cefotaxime (8.5%), ceftazidime (6%), cefepime (1.7%) and aztreonam (0.9%), whereas all isolates were susceptible to amikacin. Clinical isolates showed significant association with resistance to doripenem, imipenem and meropenem compared to non-clinical isolates. These were detected in clinical isolates with resistance phenotypes: doripenem (67.2%, 45/67), imipenem (65.9%, 54/82) and meropenem (65.2%, 30/46). Our findings showed that the MicroScan microdilution method is suitable for the detection of carbapenem resistance in both clinical (48.9-87.2%) and non-clinical (4.3-13.0%) isolates. This study revealed that isolates had relatively high carbapenem resistance, which may lead to potential treatment failure. Continued monitoring of aquatic sources with a larger sample size should be carried out to provide further insights.
在水生栖息地中普遍存在，可导致人类危及生命的败血症。然而，关于它们的抗菌药物敏感性测试（AST）概况的数据有限。因此，我们旨在使用临床（n = 94）和非临床（n = 23）分离株，通过脱水的MicroScan微量稀释法来检测它们的AST模式。对碳青霉烯耐药分离株进一步使用分子检测法筛查与碳青霉烯耐药相关的基因。这些分离株对亚胺培南（76.9%）、多利培南（62.4%）、美罗培南（41.9%）、甲氧苄啶/磺胺甲恶唑（11.1%）、头孢噻肟（8.5%）、头孢他啶（6%）、头孢吡肟（1.7%）和氨曲南（0.9%）表现出耐药性，而所有分离株对阿米卡星敏感。与非临床分离株相比，临床分离株显示出与对多利培南、亚胺培南和美罗培南耐药的显著相关性。这些在具有耐药表型的临床分离株中被检测到：多利培南（67.2%，45/67）、亚胺培南（65.9%，54/82）和美罗培南（65.2%，30/46）。我们的研究结果表明，MicroScan微量稀释法适用于检测临床（48.9 - 87.2%）和非临床（4.3 - 13.0%）分离株中的碳青霉烯耐药性。这项研究表明，分离株具有相对较高的碳青霉烯耐药性，这可能导致潜在的治疗失败。应进行更大样本量的持续监测以提供进一步的见解。
