Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan, People's Republic of China.
J Food Biochem. 2022 Sep;46(9):e14252. doi: 10.1111/jfbc.14252. Epub 2022 Jul 27.
Artemisia selengensis Turcz (AST) as a common vegetable is rich in di-caffeoylquinic acids (di-CQAs) and has been reported to possess multiple health benefits. However, whether di-CQAs from AST leaf extracts (ASTE) could alleviate gout inflammation is still unknown. Herein, this study explored the inhibitory mechanism of ASTE on gout inflammation in THP-1 macrophages. Results suggested that ASTE suppressed the secretion and mRNA levels of inflammatory cytokines including interleukin-18, interleukin-1β, interleukin-6, and tumor necrosis factor-α. Pretreatment with ASTE inhibited lipopolysaccharide-induced of IκBα degradation, p65 phosphorylation and up-regulation of Nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome proteins. Moreover, ASTE inhibited monosodium urate-induced the up-regulation of active caspase-1 and interleukin-1β, promoted nuclear factor E2-related factor2 (Nrf2) to translocate into the nucleus, reducing the generation of MSU-induced reactive oxygen species. These results suggested that ASTE alleviated gout inflammation via inhibiting NLRP3 inflammasome activation and activating Nrf2 signaling pathway. PRACTICAL APPLICATIONS: Artemisia selengensis Turcz (AST) as a common vegetable in China belongs to genus Artemisia, which are rich in di-caffeoylquinic acids. This study aimed to investigate the effect of ASTE on alleviating gout inflammation and whether NLRP3 inflammasome and Nrf2 signaling pathways are involved in the protection of ASTE against gout inflammation. Our findings are significant for developing di-CQAs from AST by-products as an effective functional food for preventing gout.
Artemisia selengensis Turcz（AST）作为一种常见的蔬菜，富含二咖啡酰奎宁酸（di-CQAs），并已被报道具有多种健康益处。然而，AST 叶提取物（ASTE）中的二咖啡酰奎宁酸是否可以缓解痛风炎症尚不清楚。本研究探讨了 ASTE 对 THP-1 巨噬细胞中痛风炎症的抑制机制。结果表明，ASTE 抑制了包括白细胞介素-18、白细胞介素-1β、白细胞介素-6 和肿瘤坏死因子-α在内的炎症细胞因子的分泌和 mRNA 水平。ASTE 预处理抑制了脂多糖诱导的 IκBα降解、p65 磷酸化和核苷酸结合寡聚结构域样受体含吡咯结构域 3（NLRP3）炎性小体蛋白的上调。此外，ASTE 抑制了单钠尿酸盐诱导的活性半胱天冬酶-1和白细胞介素-1β的上调，促进核因子 E2 相关因子 2（Nrf2）向核内易位，减少 MSU 诱导的活性氧的产生。这些结果表明，ASTE 通过抑制 NLRP3 炎性小体的激活和激活 Nrf2 信号通路来缓解痛风炎症。实际应用：中国常见的蔬菜 Artemisia selengensis Turcz（AST）属于 Artemisia 属，富含二咖啡酰奎宁酸。本研究旨在探讨 ASTE 缓解痛风炎症的作用，以及 NLRP3 炎性小体和 Nrf2 信号通路是否参与了 ASTE 对痛风炎症的保护作用。我们的研究结果为开发来自 AST 的二咖啡酰奎宁酸作为预防痛风的有效功能性食品具有重要意义。
