Anichin V F
Arkh Anat Gistol Embriol. 1978 Aug;75(8):77-83.
The otic labyrinth presents one of the most difficult objects for histological investigations as it is situated in the thick layer of the temporal bone and has a very complex structure. At the same time, a method for treating this object is not given in general hand-books on pathological techniques. The methods for histological treatment of the otic labyrinth elaborated by Wittmaack and later on modified are presented in the article. Methods for fixation, vital fixation including, decalcination, dehydratation and saturation of the objects prepared for histological investigation are described in details. Methods for the temporal bone orientation in order to obtain sections of the internal otic structures in the most advantageous plane depending on the purpose and aim of the investigation are recommended.
内耳迷路是组织学研究中最难处理的对象之一,因为它位于颞骨的厚层中,结构非常复杂。同时,一般的病理技术手册中并未给出处理该对象的方法。本文介绍了由维特马克提出并随后经过改进的内耳迷路组织学处理方法。详细描述了固定方法,包括活体固定、脱钙、脱水以及为组织学研究准备的标本的浸透方法。还推荐了根据研究目的在颞骨上确定方向的方法,以便在最有利的平面上获取内耳内部结构的切片。