Pesti Adrián, Gyömörei Csaba, Juhász Péter, Kálmán Endre, Kiss András, Kuthi Levente, Lotz Gábor, Méhes Gábor, Schaff Zsuzsa, Tiszlavicz László
1 Semmelweis Egyetem, Általános Orvostudományi Kar, Patológiai, Igazságügyi és Biztosítási Orvostani Intézet Budapest, Üllői út 93., 1091 Magyarország.
2 Pécsi Tudományegyetem, Klinikai Központ, Általános Orvostudományi Kar, Patológiai Intézet Pécs Magyarország.
Orv Hetil. 2022 Jun 19;163(25):975-983. doi: 10.1556/650.2022.32536.
Introduction: The COVID-19 (coronavirus disease 2019) caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) is associated with high mortality rates worldwide. Polymerase chain reaction (PCR) is extensively used for virus detection in both infected patients and deceased persons. PCR, however, gives no information about the localization of the virus in cells and tissues. Detection of spike and nucleocapsid proteins and viral ribonucleic acid (RNA) of the SARS-CoV-2 in situ might provide more information and aid in the discovery of the pathomechanism of cellular damage. There are several commercially available anti-spike and anti-nucleocapsid antibodies used to detect immunohistochemical reactions, though each gives different results. Objective: The goal of the present study was to compare the intensity and specificity of several anti-spike and antinucleocapsid antibodies in different dilutions in four Hungarian university departments. Method: Immunohistochemical reactions were performed on coded slides taken from infected lungs of 3 deceased and placenta samples with appropriate negative controls of formalin-fixed paraffin-embedded tissues, scanned, evaluated unanimously and analysed statistically by the assessors. Results: By comparing the intensity, dilution, background and reproducibility of the different primary antibodies, it was possible to select the antibodies with the best results. Conclusion: The antibodies selected with established dilutions can be used in further studies to detect SARS-CoV-2 proteins in surgical materials and in samples obtained during autopsy.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的2019冠状病毒病（COVID-19）在全球范围内导致了高死亡率。聚合酶链反应（PCR）被广泛用于检测感染患者和死者体内的病毒。然而，PCR无法提供病毒在细胞和组织中的定位信息。原位检测SARS-CoV-2的刺突蛋白、核衣壳蛋白和病毒核糖核酸（RNA）可能会提供更多信息，并有助于发现细胞损伤的发病机制。有几种市售的抗刺突和抗核衣壳抗体可用于检测免疫组织化学反应，不过每种抗体给出的结果都不同。目的：本研究的目的是比较匈牙利四个大学部门中几种抗刺突和抗核衣壳抗体在不同稀释度下的强度和特异性。方法：对取自3名死者感染肺部的编码切片以及胎盘样本进行免疫组织化学反应，并设置福尔马林固定石蜡包埋组织的适当阴性对照，进行扫描、由评估人员一致评估并进行统计分析。结果：通过比较不同一抗的强度、稀释度、背景和可重复性，有可能选出效果最佳的抗体。结论：选定的具有既定稀释度的抗体可用于进一步研究，以检测手术材料和尸检期间获得的样本中的SARS-CoV-2蛋白。
