Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Department of Veterinary Medicine and Epidemiology, University of California-Davis, School of Veterinary Medicine, Davis, CA.
Am J Vet Res. 2022 Jun 13;83(8):ajvr.22.01.0001. doi: 10.2460/ajvr.22.01.0001.
To compare stress markers, gastrointestinal motility, and behavioral indicators of pain between guinea pigs undergoing pneumoperitoneum with carbon dioxide (CO2) and control guinea pigs.
Fourteen 4- to 5-month-old intact female Hartley guinea pigs.
Guinea pigs were randomized to receive insufflation or serve as controls (anesthesia and abdominal catheter placement without insufflation), with 7 animals/group. Insufflated animals underwent 6 mm Hg of CO2 pneumoperitoneum for 30 minutes. Afterward, results for vital signs, blood glucose, fecal cortisol, appetite, fecal output, and behaviors (via video recording) were compared between the 2 groups.
There was no difference between groups and over time for body temperature, heart rate, fecal output in grams, pellets consumed, blood glucose, and fecal cortisol. Guinea pigs that underwent insufflation had significantly more fecal pellets at 36 hours after the procedure. Several behaviors were expressed similarly between groups and over time, such as body turns, incomplete movement, rearing, lying down, drinking, and hiding. Coprophagy occurred less often in the insufflated versus noninsufflated group at 12 h postprocedure but was similar between groups at other time points. At 60 hours after the procedure, insufflated animals spent less time squinting compared to noninsufflated animals. Other behaviors were differentially expressed over time but not between treatments.
Overall, there were no major differences in appetite, stress markers, and behaviors between insufflated and control guinea pigs. CO2 insufflation did not appear to cause undue pain or stress in guinea pigs and may be a reasonable technique to use during laparoscopy.
比较二氧化碳（CO2）气腹与对照豚鼠行气腹术时的应激标志物、胃肠动力和疼痛行为指标。
14 只 4 至 5 月龄完整的雌性 Hartley 豚鼠。
将豚鼠随机分为充气组或对照组（麻醉和腹部导管放置但不充气），每组 7 只。充气组行 6mmHg 的 CO2 气腹 30 分钟。之后，比较两组间生命体征、血糖、粪便皮质醇、食欲、粪便排出量和行为（通过视频记录）结果。
两组间和随时间变化，体温、心率、粪便排出量（克）、颗粒消耗量、血糖和粪便皮质醇均无差异。气腹组在术后 36 小时的粪便颗粒明显更多。两组间和随时间变化，某些行为相似，如身体转动、不完全运动、站立、躺下、饮水和躲藏。术后 12 小时，气腹组比非气腹组的食粪行为更少，但在其他时间点两组间无差异。术后 60 小时，气腹组比非气腹组的闭眼时间更少。其他行为随时间不同，但与处理无关。
总体而言，充气组和对照组的食欲、应激标志物和行为无明显差异。CO2 充气似乎不会给豚鼠造成过度疼痛或应激，可能是腹腔镜检查中合理的技术。
