Ulshafer R J, Spoerri P E, Allen C B, Kelley K C
Scanning Microsc. 1987 Mar;1(1):241-6.
Scanning electron microscopy was performed on cell cultures of embryonic and post-hatch chick retinas co-cultured with optic lobe neurons or in medium that had been pre-conditioned with optic lobe cells. The culture medium consisted of Eagles Basal Medium supplemented with glucose, fetal calf serum, glutamine and bicarbonate. Application of colchicine (5 micrograms/ml) to the cultures, encouraged the dissociation of retinal cell rosettes and optic lobe neuron aggregates, thereby allowing us to examine differentiation of isolated photoreceptor cells. Over time, developing photoreceptor cells gradually took on the morphological characteristics of rods and cones in the post-hatch chick: cells were polarized having a single neurite on one end of the cell and inner and outer segment-like structures on the other end. Developing cone cells elaborated an oil droplet and filopodial-like processes at the apical end of the inner segment. The latter may correspond to the calyceal processes which normally envelop the basal 1/3 of the outer segment. The sequence of events noted in vitro parallel those previously reported in vivo.
对与视叶神经元共同培养或在已用视叶细胞预处理的培养基中培养的胚胎期和孵化后雏鸡视网膜细胞培养物进行了扫描电子显微镜检查。培养基由添加了葡萄糖、胎牛血清、谷氨酰胺和碳酸氢盐的伊格尔基础培养基组成。向培养物中加入秋水仙碱（5微克/毫升），促使视网膜细胞玫瑰花结和视叶神经元聚集体解离，从而使我们能够检查分离的感光细胞的分化情况。随着时间的推移，发育中的感光细胞逐渐呈现出孵化后雏鸡中视杆和视锥细胞的形态特征：细胞呈极化状态，一端有单个神经突，另一端有内段和外段样结构。发育中的视锥细胞在内段顶端形成一个油滴和丝状伪足样突起。后者可能对应于通常包裹外段基部1/3的杯状突起。体外观察到的事件顺序与先前体内报道的一致。
