Dong Thap University, 783 Pham Huu Lau Street, Cao Lanh City, Dong Thap Province, Viet Nam.
Can J Microbiol. 2022 Sep 1;68(9):605-613. doi: 10.1139/cjm-2022-0084. Epub 2022 Jul 27.
Isoproturon (IPU) is widely used to control annual grasses and broad leaf weeds in cereal crops. In this study, four IPU-degrading bacterial strains, i.e., sp. ISP1, sp. ISP2, 4IA, and sp. ISP3, were isolated from agricultural soil. The mixed culture of four isolates completely degraded the herbicide at 100 mg/L within 10 days. During IPU degradation, several transient accumulations of the metabolites, including 3-(4-isopropylphenyl)-1-methylurea, 3-(4-isopropylphenyl)-urea, 4-isopropylaniline, and 4-toluidine, were also identified. Moreover, the inoculation of the isolated mixed culture into the soil from a mountain with no previous herbicide application increased the degradation rate by 51% of the herbicide on average. Furthermore, bioaugmentation with isolated bacteria in the soil resulted in short-term variations in bacterial structure compared to the unaugmented soil. The findings of this study were instrumental in understanding the mechanisms of pesticide breakdown and bioremediation in liquid media and soil.
异丙隆（IPU）被广泛用于控制谷物作物中的一年生杂草和阔叶杂草。在这项研究中，从农业土壤中分离出了四种能够降解异丙隆的细菌菌株，即 sp. ISP1、sp. ISP2、4IA 和 sp. ISP3。四种分离物的混合培养物在 10 天内可完全降解 100mg/L 的除草剂。在异丙隆降解过程中，还鉴定出了几种中间代谢产物的短暂积累，包括 3-(4-异丙基苯基）-1-甲基脲、3-(4-异丙基苯基）-脲、4-异丙基苯胺和 4-甲苯胺。此外，将分离出的混合培养物接种到以前未使用过除草剂的山区土壤中，平均可使除草剂的降解率提高 51%。此外，与未添加菌的土壤相比，在土壤中添加分离菌进行生物强化会导致细菌结构的短期变化。本研究的结果有助于理解农药在液体介质和土壤中的降解和生物修复机制。
