School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China.
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Molecules. 2022 Jul 24;27(15):4727. doi: 10.3390/molecules27154727.
For hundreds of years, (DC.) Koidz. (AK) has been widely used as a treatment for spleen and stomach diseases in China. The AK polysaccharides (AKPs) have been thought to be the important bioactive components. In this stud, the impacts of different extraction methods were analyzed. The differences between AKPs extracted by hot water extraction (HWE), AKPs extracted by ultrasonic extraction (UAE), and AKPs extracted by enzyme extraction (EAE) were compared in terms of yield, total carbohydrate content, molecular weight distribution, monosaccharide composition, and synergistic activity of the AKPs with apatinib were determined. The results indicated that the yield of the polysaccharide obtained from HWE was higher than that of UAE and EAE. However, activity assays indicated that UAE-AKPs and HWE-AKPs enhanced apoptosis of human gastric cancer cells (SGC-7901) treated with apatinib and UAE-AKPs showed the strongest synergistic activities. This is also in agreement with the fact that UAE-AKPs have a smaller molecular weight, β-configuration, and higher galactose content. These findings suggested that UAE is an efficient and environmentally friendly method for producing new polysaccharides from (DC.) Koidz. for the development of natural synergist and for the treatment of gastric cancer.
数百年来，（DC.）魁蚶（AK）在中国一直被广泛用作治疗脾胃疾病的药物。AK 多糖（AKPs）被认为是重要的生物活性成分。在这项研究中，分析了不同提取方法的影响。比较了热水提取（HWE）、超声提取（UAE）和酶提取（EAE）提取的 AKPs 在产率、总糖含量、分子量分布、单糖组成以及与阿帕替尼协同活性方面的差异。结果表明，HWE 提取的多糖产率高于 UAE 和 EAE。然而，活性测定表明，UAE-AKPs 和 HWE-AKPs 增强了阿帕替尼处理的人胃癌细胞（SGC-7901）的凋亡，并且 UAE-AKPs 表现出最强的协同活性。这也与事实相符，即 UAE-AKPs 具有较小的分子量、β 构型和更高的半乳糖含量。这些发现表明，UAE 是从（DC.）魁蚶生产新型多糖的一种有效且环保的方法，可用于开发天然协同剂和治疗胃癌。
