合生元皮肤提取物和 K12 抑制物种生物膜形成。
Synbiotic skin extract and K12 inhibit species biofilm formation.
机构信息
Cluster of Cancer Research Initiative IIUM (COCRII), International Islamic University Malaysia, Kuantan, Pahang, Malaysia.
Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan, Pahang, Malaysia.
出版信息
Biofouling. 2022 Jul;38(6):614-627. doi: 10.1080/08927014.2022.2105142. Epub 2022 Jul 28.
This study aimed to determine the effect of synbiotic skin extract (MASE) and K12 (K12) on species biofilm formation. Liquid chromatography quadrupole time-of-flight (LC-Q-TOF-MS) was conducted to characterize MASE. To determine the effect of synbiotic on biofilm, 200 µL of RPMI-1640 containing , K12, and MASE were pipetted into the same well and incubated at 37 °C for 72 h. A similar protocol was repeated with K12 or MASE to determine the probiotic and prebiotic effects, respectively. Dimorphism, biofilm biomass, and total cell count (TCC) were determined. A total of 60 compounds were detected in MASE. (ALT5) and exhibited the highest reduction in biofilm biomass when co-cultured with prebiotic (77.70 ± 7.67%) and synbiotic (97.73 ± 0.28%), respectively. All spp. had decreased TCC and hyphae when co-cultured with synbiotic. In conclusion, MASE and K12 inhibit biofilm formation.
本研究旨在确定共生皮肤提取物 (MASE) 和 K12 (K12) 对 种生物膜形成的影响。采用液相色谱四极杆飞行时间质谱 (LC-Q-TOF-MS) 对 MASE 进行表征。为了确定共生对生物膜的影响，将含有 、K12 和 MASE 的 200μL RPMI-1640 分别吸入同一孔中，在 37°C 下孵育 72 小时。用 K12 或 MASE 重复相同的方案，以分别确定益生菌和益生元的作用。测定二态性、生物膜生物量和总细胞计数 (TCC)。在 MASE 中检测到 60 种化合物。与益生菌 (77.70±7.67%) 和共生菌 (97.73±0.28%) 共培养时，ALT5 和 表现出最高的生物膜生物量降低。所有 种。与共生菌共培养时，TCC 和菌丝减少。总之，MASE 和 K12 抑制 生物膜形成。
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