Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China.
Anal Chem. 2022 Aug 9;94(31):11089-11095. doi: 10.1021/acs.analchem.2c02312. Epub 2022 Jul 28.
Cancer is a health threat worldwide, and it is urgent to develop more sensitive cancer detection methods. Herein, a polarity-sensitive cell membrane probe (named ) was developed for detecting cancer cells and tumors sensitively and selectively at the cell membrane level. The probe shows a strong polarity-dependent fluorescence and excellent cell membrane targeting ability to visualize cell membrane with red fluorescence with a non-washing process. Notably, can selectively light up the tumor cell membranes, which reveals that cancer cell membranes have lower polarity than normal cell membranes. The giant unilamellar vesicle model and cell imaging studies proved this. Moreover, can effectively and selectively light up tumors. Overall, this work demonstrates that the polarity of the tumor cell membrane is quite different to normal cell membranes, and based on this, sensitive membrane probes can be developed to selectively visualize cancer cells and tumors, which opens up a new way for tumor diagnosis at the cellular level.
癌症是全球范围内的健康威胁，因此迫切需要开发更灵敏的癌症检测方法。在此，我们开发了一种对极性敏感的细胞膜探针（命名为 ），用于在细胞膜水平上灵敏和选择性地检测癌细胞和肿瘤。该探针表现出很强的极性依赖性荧光和优异的细胞膜靶向能力，无需洗涤即可将细胞膜可视化呈现红色荧光。值得注意的是， 可以选择性地点亮肿瘤细胞膜，这表明癌细胞膜的极性低于正常细胞膜。巨大的单层囊泡模型和细胞成像研究证明了这一点。此外， 还可以有效地和选择性地点亮肿瘤。总的来说，这项工作表明肿瘤细胞膜的极性与正常细胞膜有很大的不同，基于这一点，可以开发出对细胞膜具有高选择性的灵敏膜探针，以选择性地可视化癌细胞和肿瘤，这为细胞水平的肿瘤诊断开辟了新途径。
