Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an City, 271018, Shandong Province, China.
Institute of Animal Science and Veterinary Medicine, Yantai Academy of Agricultural Sciences, Yantai City, 265599, Shandong Province, China.
Genes Genomics. 2022 Oct;44(10):1149-1158. doi: 10.1007/s13258-022-01271-8. Epub 2022 Jul 28.
Oxidative stress is one of the most important factors affecting large-scale breeding, especially the performance of pigs. Oxidative stress plays a role by affecting various genes in pigs, which can cause serious body damage, functional degradation and reduce production performance.
The purpose of this study was to investigate the effect of Toll like receptor 9 (TLR9) pathway on IPEC-J2 cells under oxidative stress and to provide reference for the growth development of Dapulian pigs.
In this study, Diquat was used as a source of oxidative stress to study the effects on Dapulian pigs by detecting relevant indicators. Then the IPEC-J2 cells were selected to verify the TLR9 signaling pathway in oxidative stress.
Compared with the control group, superoxide dismutase (SOD) in experimental group decreased significantly, malondialdehyde (MDA) was significantly increased, accompanied by inflammatory reaction, and inflammatory factors were significantly increased in the experimental group. Oxidative stress model was constructed by H2O2 incubating IPEC-J2 cells. The interference and overexpression vectors of TLR9 and myeloid differentiation primary response protein 88 (MyD88) were constructed to detect the activity of antioxidant enzymes and related proteins. The results showed that overexpression of TLR9 enhanced the activity of antioxidant enzymes, decreased the secretion of inflammatory factors, and decreased the activity of MDA，reactive oxygen species (ROS); the results were opposite after TLR9 interference. This study also showed that HO can activate the nuclear factor-κB (NF-κB) pathway and promote the translocation of NF-κB into the nucleus. After co-transfection with TLR9 and MyD88, the results showed that TLR9 regulated the expression of NF-κB through MyD88.
The study showed that TLR9 pathway had a significant positive effect on antioxidant.
氧化应激是影响大规模繁殖的最重要因素之一，尤其是猪的性能。氧化应激通过影响猪体内的各种基因发挥作用，这可能导致严重的身体损伤、功能下降并降低生产性能。
本研究旨在探讨 Toll 样受体 9（TLR9）通路在氧化应激下对 IPEC-J2 细胞的影响，为杜洛克猪的生长发育提供参考。
本研究以百草枯为氧化应激源，通过检测相关指标研究其对杜洛克猪的影响。然后选择 IPEC-J2 细胞验证氧化应激下 TLR9 信号通路。
与对照组相比，实验组超氧化物歧化酶（SOD）显著降低，丙二醛（MDA）显著升高，伴有炎症反应，实验组炎症因子明显增加。用 H2O2 孵育 IPEC-J2 细胞构建氧化应激模型。构建 TLR9 和髓样分化初级反应蛋白 88（MyD88）的干扰和过表达载体，检测抗氧化酶和相关蛋白的活性。结果表明，TLR9 过表达增强了抗氧化酶的活性，降低了炎症因子的分泌，降低了 MDA、活性氧（ROS）的活性；TLR9 干扰后结果相反。本研究还表明，HO 可以激活核因子-κB（NF-κB）通路，促进 NF-κB 向核内转位。共转染 TLR9 和 MyD88 后，结果表明 TLR9 通过 MyD88 调节 NF-κB 的表达。
该研究表明 TLR9 通路对抗氧化具有显著的正向作用。
