School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
Food Chem. 2022 Dec 1;396:133743. doi: 10.1016/j.foodchem.2022.133743. Epub 2022 Jul 19.
A novel green alternative vortex-assisted emulsification liquid-liquid microextraction based on a new hydrophobic deep eutectic solvent (DES) was developed to extract tetracyclines in infant formulas. Five new ternary DESs were synthesized and tested in this regard. Among them [thymol]:[ethylene glycol]:[benzyl alcohol] (2:2:1) DES demonstrated the highest extraction efficiency. The analyzes were performed by HPLC. The DES was characterized by attenuated total reflectance-FTIR, nuclear magnetic resonance spectroscopy, and octanol-water partition coefficient. The effect of type, mole ratio, and volume of DES, dispersing agent, pH, and ionic strength was studied. The limits of quantification and linear range were 3-9.32 and 3-500 μg kg, respectively. The method was applied to detect tetracyclines in infant formulas, and the recoveries were obtained as 68.9-102.0 % with relative standard deviations < 9 %. The procedure greenness was assessed using the Analytical Eco-Scale. This paper represents the first application of a novel ternary DES to analyze tetracyclines in infant formulas.
一种新型的绿色替代方法——基于新型疏水深共晶溶剂 (DES) 的涡旋辅助乳化液液微萃取已被开发用于从婴儿配方食品中提取四环素。在这方面合成并测试了五种新的三元 DES。其中[百里酚]：[乙二醇]：[苯甲醇]（2:2:1）DES 表现出最高的萃取效率。分析采用 HPLC 进行。DES 通过衰减全反射-FTIR、核磁共振波谱和辛醇-水分配系数进行了表征。研究了 DES 的类型、摩尔比和体积、分散剂、pH 值和离子强度的影响。定量限和线性范围分别为 3-9.32 和 3-500μg kg。该方法用于检测婴儿配方食品中的四环素，回收率为 68.9-102.0%，相对标准偏差 < 9%。使用分析生态规模评估了该程序的绿色度。本文代表了将新型三元 DES 首次应用于分析婴儿配方食品中的四环素。
