College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Wuhan Academy of Agricultural Sciences, Wuhan, 430070, China.
Anal Bioanal Chem. 2023 Jul;415(17):3581-3592. doi: 10.1007/s00216-023-04749-w. Epub 2023 May 27.
Enrichment for the detection of quinolone residues is usually cumbersome and requires large amounts of toxic organic reagents. Therefore, this study synthesized a low-toxicity hydrophobic deep eutectic solvent (DES) with DL-menthol and p-cresol, which was then characterized by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and thermal analysis. A simple and rapid vortex-assisted liquid-liquid microextraction method was developed based on this DES for the extraction of eight quinolones from cattle urine. The optimal extraction conditions were screened by examining the DES volume, extraction temperature, vortex time, and salt concentration. Under the optimal conditions, the linear ranges of the eight quinolones were 1 ~ 100 μg/L with good linearity (r was 0.998 ~ 0.999), and the limits of detection and quantification were 0.08 ~ 0.30 μg/L and 0.27 ~ 0.98 μg/L, respectively. The average extraction recoveries of spiked cattle urine samples were 70.13 ~ 98.50% with relative standard deviations below 13.97%. This method can provide a reference for the pre-treatment of quinolone residue detection.
喹诺酮类残留的检测通常很繁琐，需要大量有毒的有机试剂。因此，本研究合成了一种低毒疏水性深共晶溶剂（DES），由 DL-薄荷醇和对甲酚组成，并用傅里叶变换红外光谱、核磁共振和热分析对其进行了表征。基于这种 DES，开发了一种简单快速的涡旋辅助液-液微萃取方法，用于从牛尿中提取 8 种喹诺酮类药物。通过考察 DES 体积、萃取温度、涡旋时间和盐浓度，筛选出最佳萃取条件。在最佳条件下，8 种喹诺酮类药物的线性范围为 1 ～ 100μg/L，线性良好（r 为 0.998 ～ 0.999），检出限和定量限分别为 0.08 ～ 0.30μg/L 和 0.27 ～ 0.98μg/L。加标牛尿样品的平均提取回收率为 70.13 ％～ 98.50％，相对标准偏差低于 13.97％。该方法可为喹诺酮类残留检测的前处理提供参考。
