Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, South Korea.
Department of Electrical Engineering, Korea National University of Transportation, Chungju 27469, South Korea.
Bioelectrochemistry. 2022 Oct;147:108214. doi: 10.1016/j.bioelechem.2022.108214. Epub 2022 Jul 21.
Heavy metal ions are toxic to humans and can further interact with amyloid in the human body to produce amyloid plaques, which disrupt neurotransmitter function and are linked to Alzheimer's and Parkinson's diseases. In this study, we developed an amyloid oligomer-reduced graphene oxide composite (AOrGOC) electrochemical sensor for effective heavy metal ion detection based on square-wave anodic stripping voltammetry. The reactivity between amyloids and heavy metal ions was studied by analyzing the stripping current for different amyloids (lysozyme, bovine serum albumin, and β-lactoglobulin) and amyloid growth types (monomers, oligomers, and fibrils). Reduced graphene oxide was used to improve the sensitivity of the sensor. The AOrGOC sensor exhibited the detection limits of 86.0 and 9.5 nM for Cd and Pb, respectively, and selectively detected Cd and Pb over other heavy metal ions. The AOrGOC sensor also detected Cd and Pb in human plasma, thus exhibiting its potential as a biosensor. This study not only promoted our fundamental understanding of amyloids and the detection of heavy metal ions using amyloids, but also provided valuable insights into amyloid-based electrochemical sensors.
重金属离子对人体有毒，并且可以进一步与人体内的淀粉样蛋白相互作用，产生淀粉样斑块，破坏神经递质功能，与阿尔茨海默病和帕金森病有关。在这项研究中，我们基于方波阳极溶出伏安法开发了一种用于有效检测重金属离子的淀粉样寡聚体还原氧化石墨烯复合材料（AOrGOC）电化学传感器。通过分析不同淀粉样蛋白（溶菌酶、牛血清白蛋白和β-乳球蛋白）和淀粉样蛋白生长类型（单体、寡聚体和原纤维）的溶出电流，研究了淀粉样蛋白与重金属离子之间的反应性。还原氧化石墨烯用于提高传感器的灵敏度。AOrGOC 传感器对 Cd 和 Pb 的检测限分别为 86.0 和 9.5 nM，并且对 Cd 和 Pb 具有选择性检测能力，超过了其他重金属离子。AOrGOC 传感器还检测了人血浆中的 Cd 和 Pb，因此表现出作为生物传感器的潜力。这项研究不仅促进了我们对淀粉样蛋白的基本理解以及使用淀粉样蛋白检测重金属离子，而且为基于淀粉样蛋白的电化学传感器提供了有价值的见解。
