Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, PR China.
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, PR China.
Res Vet Sci. 2022 Dec 20;152:10-19. doi: 10.1016/j.rvsc.2022.07.011. Epub 2022 Jul 22.
Avian pathogenic Escherichia coli (APEC) can cause the acute and sudden death of poultry, which leads to serious economic losses in the poultry industry. Biofilm formation contributes to the persistence of bacterial infection, drug resistance, and resistance to diverse environmental stress. Many transcription regulators in APEC play an essential role in the formation of biofilm and could provide further insights into APEC pathogenesis. YjjQ has an important role in the pathogenicity of bacteria by regulating the expression of virulence factors, such as flagellar and iron uptake. However, YjjQ regulates other virulence factors, and their role in the overall regulatory network is unclear. Here, we further evaluate the function of YjjQ on APEC biofilm formation and motility. In this study, we successfully constructed mutant (AE27∆yjjQ) and complement (AE27ΔyjjQ-comp) strains of the wild-type strain AE27. Inactivation of the yjjQ gene significantly increased biofilm-forming ability in APEC. Scanning electron microscopy showed that the biofilm formation of the AE27 was single-layered and flat, whereas that of the AE27∆yjjQ had a porous three-dimensional structure. Moreover, the deletion of the yjjQ gene inhibited the motility of APEC. RNA-sequencing was used to further investigate the regulatory mechanism of YjjQ in APEC. The results indicate that YjjQ regulates biofilm formation and flagellar genes in AE27∆yjjQ. RT-qPCR shows that YjjQ affects the transcriptional levels of genes, including flagella genes (flhD, flhC and flgE), and biofilm formation genes (pstA, uhpC, nikD, and ygcS). These results confirm that the transcription regulator YjjQ is involved in APEC biofilm formation and motility, and provide new evidence for the prevention and control of APEC.
禽致病性大肠杆菌（APEC）可引起家禽的急性和突然死亡，导致家禽业遭受严重的经济损失。生物膜的形成有助于细菌感染的持续存在、耐药性和对各种环境压力的抵抗力。APEC 中的许多转录调节因子在生物膜的形成中起着重要作用，并为 APEC 发病机制提供了进一步的见解。YjjQ 通过调节鞭毛和铁摄取等毒力因子的表达，在细菌的致病性中起着重要作用。然而，YjjQ 调节其他毒力因子，其在整个调节网络中的作用尚不清楚。在这里，我们进一步评估了 YjjQ 对 APEC 生物膜形成和运动性的功能。在本研究中，我们成功构建了野生型菌株 AE27 的突变（AE27∆yjjQ）和互补（AE27ΔyjjQ-comp）菌株。yjjQ 基因的失活显著增加了 APEC 的生物膜形成能力。扫描电子显微镜显示，AE27 的生物膜形成是单层和平坦的，而 AE27∆yjjQ 的生物膜形成具有多孔的三维结构。此外，yjjQ 基因的缺失抑制了 APEC 的运动性。RNA-seq 进一步研究了 YjjQ 在 APEC 中的调节机制。结果表明，YjjQ 调节 AE27∆yjjQ 中的生物膜形成和鞭毛基因。RT-qPCR 显示，YjjQ 影响包括鞭毛基因（flhD、flhC 和 flgE）和生物膜形成基因（pstA、uhpC、nikD 和 ygcS）在内的基因的转录水平。这些结果证实了转录调节因子 YjjQ 参与了 APEC 的生物膜形成和运动性，为 APEC 的预防和控制提供了新的证据。
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