Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Poult Sci. 2022 Sep;101(9):102011. doi: 10.1016/j.psj.2022.102011. Epub 2022 Jun 16.
Bird death is often caused by renal lesions induced by chemicals. The avian kidney has a renal portal system with significant blood flow that is sensitive to many chemicals. However, early avian biomarkers for kidney injury are yet to be identified. This study aimed to identify novel renal biomarkers. Acute kidney injury (AKI) can be divided into acute interstitial nephritis (AIN) and acute tubular necrosis (ATN). A chicken model of kidney damage was created by an injection of diclofenac or cisplatin, which caused either AIN or ATN, respectively. Microarray analysis was performed to profile the gene expression patterns in the chickens with nephropathy. A gene enrichment analysis suggested that the genes related to responses to external stimuli showed expression changes in both AIN and ATN. However, hierarchical clustering analyses suggested that gene expression patterns differed between AIN and ATN, and the number of biomarkers relating to renal damage was low. To identify early biomarkers for nephropathy, we focused on genes that were induced at various levels of renal damage. The gene, vanin-1 (VNN1) was highly induced in the early stages of renal damage. A quantitative real-time PCR analysis supported this finding. These results suggest VNN1 could be a useful early biomarker of kidney injury in avian species.
鸟类死亡通常是由化学物质引起的肾脏病变导致的。禽类的肾脏具有肾门静脉系统，其血流量很大，对许多化学物质都很敏感。然而，目前尚未确定早期的鸟类肾脏损伤生物标志物。本研究旨在寻找新的肾脏生物标志物。急性肾损伤（AKI）可分为急性间质性肾炎（AIN）和急性肾小管坏死（ATN）。通过注射双氯芬酸或顺铂在鸡中建立肾脏损伤模型，分别导致 AIN 或 ATN。通过微阵列分析对患有肾病的鸡的基因表达模式进行分析。基因富集分析表明，与对外界刺激的反应相关的基因在 AIN 和 ATN 中均发生了表达变化。然而，层次聚类分析表明，AIN 和 ATN 之间的基因表达模式存在差异，并且与肾脏损伤相关的生物标志物数量较少。为了确定肾病的早期生物标志物，我们专注于在不同程度的肾脏损伤中诱导的基因。基因，Vanin-1（VNN1）在肾脏损伤的早期阶段高度诱导。定量实时 PCR 分析支持了这一发现。这些结果表明 VNN1 可能是禽类肾脏损伤的有用早期生物标志物。
