Fair P H, Balthrop J E, Wade J L, Braddon-Galloway S
Toxicol Lett. 1987 May;36(3):213-20. doi: 10.1016/0378-4274(87)90188-3.
The effect of methylmercury and thiol complexes of methylmercury on inhibition of protein synthesis was evaluated. Mice were injected (i.p.) with the following treatments: methylmercuric chloride, methylmercury-glutathione, methylmercury-cysteinylglycine and control (vehicle) for 10 days. Ten animals from each group were injected with [14C]leucine 90 min prior to death. The brains were removed and the extracted protein was subjected to liquid scintillation analysis. Mice receiving the methylmercury and methylmercury-glutathione treatments exhibited significantly greater weight loss than the control while the methylmercury-cysteinylglycine treatment was not significantly different than the control. Incorporation of [14C]leucine into brain protein was significantly depressed in the methylmercury (81% of control) and the methylmercury-glutathione (79% of control) treatments. Protein synthesis in mice receiving the methylmercury-cysteinylglycine complex although not significantly different than the methylmercury treatments was only 92% of the control mice.
评估了甲基汞及其硫醇配合物对蛋白质合成抑制的影响。给小鼠腹腔注射以下处理药物：氯化甲基汞、甲基汞 - 谷胱甘肽、甲基汞 - 半胱氨酰甘氨酸以及对照（赋形剂），持续10天。每组10只动物在处死前90分钟注射[¹⁴C]亮氨酸。取出大脑，对提取的蛋白质进行液体闪烁分析。接受甲基汞和甲基汞 - 谷胱甘肽处理的小鼠体重减轻明显大于对照组，而甲基汞 - 半胱氨酰甘氨酸处理组与对照组无显著差异。在甲基汞处理组（为对照组的81%）和甲基汞 - 谷胱甘肽处理组（为对照组的79%）中，[¹⁴C]亮氨酸掺入脑蛋白的量显著降低。接受甲基汞 - 半胱氨酰甘氨酸配合物处理的小鼠，其蛋白质合成虽然与甲基汞处理组无显著差异，但仅为对照小鼠的92%。
