[14C]亮氨酸在经甲基汞及其硫醇络合物处理的小鼠脑蛋白中的体内掺入情况。

In vivo incorporation of [14C]leucine into brain protein of mice treated with methylmercury and thiol complexes of methylmercury.

作者信息

Fair P H, Balthrop J E, Wade J L, Braddon-Galloway S

出版信息

Toxicol Lett. 1987 May;36(3):213-20. doi: 10.1016/0378-4274(87)90188-3.

Abstract

The effect of methylmercury and thiol complexes of methylmercury on inhibition of protein synthesis was evaluated. Mice were injected (i.p.) with the following treatments: methylmercuric chloride, methylmercury-glutathione, methylmercury-cysteinylglycine and control (vehicle) for 10 days. Ten animals from each group were injected with [14C]leucine 90 min prior to death. The brains were removed and the extracted protein was subjected to liquid scintillation analysis. Mice receiving the methylmercury and methylmercury-glutathione treatments exhibited significantly greater weight loss than the control while the methylmercury-cysteinylglycine treatment was not significantly different than the control. Incorporation of [14C]leucine into brain protein was significantly depressed in the methylmercury (81% of control) and the methylmercury-glutathione (79% of control) treatments. Protein synthesis in mice receiving the methylmercury-cysteinylglycine complex although not significantly different than the methylmercury treatments was only 92% of the control mice.

摘要

评估了甲基汞及其硫醇配合物对蛋白质合成抑制的影响。给小鼠腹腔注射以下处理药物：氯化甲基汞、甲基汞 - 谷胱甘肽、甲基汞 - 半胱氨酰甘氨酸以及对照（赋形剂），持续10天。每组10只动物在处死前90分钟注射[¹⁴C]亮氨酸。取出大脑，对提取的蛋白质进行液体闪烁分析。接受甲基汞和甲基汞 - 谷胱甘肽处理的小鼠体重减轻明显大于对照组，而甲基汞 - 半胱氨酰甘氨酸处理组与对照组无显著差异。在甲基汞处理组（为对照组的81%）和甲基汞 - 谷胱甘肽处理组（为对照组的79%）中，[¹⁴C]亮氨酸掺入脑蛋白的量显著降低。接受甲基汞 - 半胱氨酰甘氨酸配合物处理的小鼠，其蛋白质合成虽然与甲基汞处理组无显著差异，但仅为对照小鼠的92%。